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Bioelectrochemical sulphate reduction on batch reactors: Effect of inoculum-type and applied potential on sulphate consumption and pH
Bioelectrochemistry ( IF 5 ) Pub Date : 2017-08-25 , DOI: 10.1016/j.bioelechem.2017.08.006
Manuel A. Gacitúa , Enyelbert Muñoz , Bernardo González

Microbial electrolysis batch reactor systems were studied employing different conditions, paying attention on the effect that biocathode potential has on pH and system performance, with the overall aim to distinguish sulphate reduction from H2 evolution. Inocula from pure strains (Desulfovibrio paquesii and Desulfobacter halotolerans) were compared to a natural source conditioned inoculum. The natural inoculum possess the potential for sulphate reduction on serum bottles experiments due to the activity of mutualistic bacteria (Sedimentibacter sp. and Bacteroides sp.) that assist sulphate-reducing bacterial cells (Desulfovibrio sp.) present in the consortium. Electrochemical batch reactors were monitored at two different potentials (graphite-bar cathodes poised at − 900 and − 400 mV versus standard hydrogen electrode) in an attempt to isolate bioelectrochemical sulphate reduction from hydrogen evolution. At − 900 mV all inocula were able to reduce sulphate with the consortium demonstrating superior performance (SO42 − consumption: 25.71 g m− 2 day− 1), despite the high alkalinisation of the media. At − 400 mV only the pure Desulfobacter halotolerans inoculated system was able to reduce sulphate (SO42 − consumption: 17.47 g m− 2 day− 1) and, in this potential condition, pH elevation was less for all systems, confirming direct (or at least preferential) bioelectrochemical reduction of sulphate over H2 production.



中文翻译:

分批反应器中生物电化学法硫酸盐的还原:接种物类型和施加电势对硫酸盐消耗和pH的影响

在不同条件下研究了微生物电解间歇反应器系统,注意生物阴极电势对pH和系统性能的影响,总体目的是区分硫酸盐还原与H 2释放。将纯菌株(Desulfovibrio paquesiiDesulfobacter halotolerans)的接种物与天然来源的条件接种物进行了比较。由于互助细菌(Sedimentibacter sp。和Bacteroides sp。)的活性有助于减少硫酸盐的细菌细胞(Desulfovibrio),天然接种物在血清瓶实验中具有硫酸盐还原的潜力。sp。)存在于财团中。电化学分批反应器在两个不同的电势下进行监控(相对于标准氢电极,石墨棒阴极放置在-900和-400 mV下),以试图将生化硫酸盐的还原反应与氢气的释放隔离开来。尽管培养基的碱化程度很高,但在− 900 mV的情况下,所有接种物均能与财团还原硫酸盐,表现出优异的性能(SO 4 2 −消耗量:25.71 g m − 2 天-1)。在− 400 mV时,仅纯卤化脱硫杆菌接种系统能够还原硫酸盐(SO 4 2 −消耗量:17.47 g m − 2 天− 1),并且在此潜在条件下,所有系统的pH升高均较小,这证实了硫酸盐与H 2产生相比直接(或至少优先)发生了生物电化学还原。

更新日期:2017-08-25
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