Shewanella species respire using iron and manganese oxides as well as electrodes as solid terminal electron acceptors. Shewanella oneidenis MR-1 exploits mediated as well as direct extracellular electron transfer (EET) modes to transfer electrons at different formal potentials. These different EET modes at different potentials may utilise alternate electron transfer pathways. Therefore, we investigated how different anode potentials, providing different maximum microbial energy gains impacted S. oneidensis microbial physiology. Using quantitative proteomics, comparative analysis of the cellular variations to different anode potentials was performed. A label-free proteomic mass spectrometric analysis method, SWATH-MS, was used to gather quantitative information to determine physiological changes of Shewanella oneidensis MR-1 grown at different anodic potentials. S. oneidensis was cultured and grown in electrochemical cells at the set anode potentials of + 0.71 V, + 0.21 V & − 0.19 V versus SHE reference electrode, while the current production was monitored. At maximum current, electrodes were removed and whole-cell proteins extracted. Subsequent SWATH-MS analysis revealed information on 740 identified proteins across the three electrode potentials. For the first time, we show the abundance of S. oneidensis electron transfer proteins differs with electrode potential.

希瓦氏菌利用铁和锰的氧化物以及电极作为固体末端电子受体来呼吸。Shewanella oneidenis MR-1利用介导的以及直接的细胞外电子转移（EET）模式来转移处于不同形式电位的电子。这些处于不同电势的不同EET模式可以利用其他电子转移途径。因此，我们研究如何不同的阳极电势，提供了不同的影响最大微生物的能量收益小号。奥尼迪斯微生物生理学。使用定量蛋白质组学，对不同阳极电位的细胞变异进行了比较分析。使用无标记蛋白质组质谱分析方法SWATH-MS收集定量信息，以确定在不同阳极电势下生长的Shewanella oneidensis MR-1的生理变化。小号。奥尼迪斯在SHE参比电极上，在设定的阳极电势为+ 0.71 V，+ 0.21 V＆− 0.19 V的条件下，在电化学电池中进行培养和生长。在最大电流下，移除电极并提取全细胞蛋白质。随后的SWATH-MS分析揭示了跨越三个电极电位的740种已鉴定蛋白的信息。第一次，我们显示了S的丰度。oneidensis电子转移蛋白随电极电位的不同而不同。