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Study of lipid peroxidation and ascorbic acid protective role in large unilamellar vesicles from a new electrochemical performance
Bioelectrochemistry ( IF 4.8 ) Pub Date : 2017-12-06 , DOI: 10.1016/j.bioelechem.2017.12.003
M. Fátima Barroso , M. Alejandra Luna , Fernando Moyano , Cristina Delerue-Matos , N. Mariano Correa , Patricia G. Molina

In this contribution an electrochemical study is described for the first time of lipid peroxidation and the role of antioxidant on lipid protection using large unilamellar vesicles (LUVs). In order to simulate the cell membrane, LUVs composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) were used. A vesicle-modified electrode was constructed by immobilizing DOPC LUVs onto carbon paste electrodes (CPEs). Lipid peroxidation was studied electrochemically by incubating the vesicle-modified electrodes with hydroxyl (HO) radicals generated via the Fenton reaction. Oxidative damage induced by HO was verified by using square wave voltammetry (SWV) and was indirectly measured by the increase of electrochemical peak current to [Fe(CN)6]4 − which was used as the electrochemical label. Ascorbic acid (AA) was used as the antioxidant model in order to study its efficacy on free radical scavenging. The decrease of the electrochemical signal confirms the protective key role promoted by AA in the prevention of lipid peroxidation in vesicles. Through microscopy, it was possible to observe morphologic modification on vesicle structures after lipid peroxidation in the presence or absence of AA.



中文翻译:

从新的电化学性能研究脂质过氧化和抗坏血酸在大单层囊泡中的保护作用

在这项贡献中,首次进行了脂质过氧化的电化学研究,以及抗氧化剂在使用大单层囊泡(LUV)的脂质保护中的作用。为了模拟细胞膜,使用了由1,2-二油酰基-sn-甘油-3-磷酸胆碱(DOPC)组成的LUV。通过将DOPC LUV固定在碳糊电极(CPE)上来构建囊泡修饰的电极。通过将囊泡修饰的电极与Fenton反应产生的羟基(HO )进行孵育,对脂质过氧化进行了电化学研究。使用方波伏安法(SWV)验证了HO引起的氧化损伤,并通过电化学峰电流增加到[Fe(CN)6 ] 4-间接测量了氧化损伤用作电化学标记。为了研究抗坏血酸清除自由基的功效,使用抗坏血酸(AA)作为抗氧化剂模型。电化学信号的减少证实了AA在预防小泡脂质过氧化中所起的保护关键作用。通过显微镜,可以在存在或不存在AA的情况下观察脂质过氧化后囊泡结构的形态学改变。

更新日期:2017-12-06
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