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Aspects on mediated glucose oxidation at a supported cubic phase
Bioelectrochemistry ( IF 5 ) Pub Date : 2017-06-22 , DOI: 10.1016/j.bioelechem.2017.06.010
Mahdi Shahmohammadi Aghbolagh , Mohammad Yaser Khani Meynaq , Kenichi Shimizu , Britta Lindholm-Sethson

A supported liquid crystalline cubic phase housing glucose oxidase on an electrode surface has been suggested as bio-anode in a biofuel. The purpose of this investigation is to clarify some aspect on the mediated enzymatic oxidation of glucose in such a bio-anode where the mediator ferrocene-carboxylic acid and glucose were dissolved in the solution. The enzyme glucose oxidase was housed in the water channels of the mono-olein cubic phase.

The system was investigated with cyclic voltammetry at different scan rates and the temperature was varied between 15 °C and 30 °C. The diffusion coefficient of the mediator and also the film resistance was estimated showing a large decrease in the mass-transport properties as the temperature was decreased. The current from mediated oxidation of glucose at the electrode surface increased with decreasing film thickness.

The transport of the mediator in the cubic phase was the rate-limiting step in the overall reaction, where the oxidation of glucose took place at the outer surface of the cubic phase.



中文翻译:

负载立方相介导的葡萄糖氧化的方面

已经提出在电极表面上容纳葡萄糖氧化酶的负载液晶立方相作为生物燃料中的生物阳极。该研究的目的是阐明在这种生物阳极中介导的葡萄糖的酶促氧化的某些方面,在该生物阳极中,调节剂二茂铁-羧酸和葡萄糖溶解在溶液中。葡萄糖氧化酶被容纳在单油精立方相的水通道中。

用循环伏安法在不同的扫描速率下对系统进行了研究,温度在15°C和30°C之间变化。估计介体的扩散系数以及膜电阻显示出随着温度降低,传质特性大大降低。电极表面的葡萄糖介导氧化电流随着膜厚度的减小而增加。

介质在立方相中的运输是整个反应的限速步骤,其中葡萄糖的氧化发生在立方相的外表面。

更新日期:2017-06-22
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