The non-haem Fe(II) and 2-oxoglutarate (2OG)-dependent oxygenases belong to a superfamily of structurally-related enzymes that play important biological roles in plants, microorganisms and animals. Structural, mechanistic and functional studies of 2OG oxygenases require efficient and effective biophysical tools. Nuclear magnetic resonance (NMR) spectroscopy is a useful tool to study this enzyme superfamily. It has been applied to obtain information about enzyme kinetics, identify and characterise 2OG oxygenase-catalysed oxidation products, elucidate the catalytic mechanism, monitor ligand binding and study protein dynamics. This review summarises the types of information that NMR spectroscopy can provide in the studies of 2OG oxygenases, highlights the advantages of the technique and describes its drawbacks.

非血红素Fe（II）和2-氧戊二酸酯（2OG）依赖性加氧酶属于结构相关酶的超家族，这些酶在植物，微生物和动物中起着重要的生物学作用。2OG氧化酶的结构，机理和功能研究需要有效的生物物理工具。核磁共振（NMR）光谱是研究此酶超家族的有用工具。它已被用于获得有关酶动力学的信息，鉴定和表征2OG加氧酶催化的氧化产物，阐明催化机理，监测配体结合并研究蛋白质动力学。这篇综述总结了NMR光谱学在2OG加氧酶研究中可以提供的信息类型，强调了该技术的优点并描述了其缺点。