The effects of different lipolytic enzymes on the structure of lipid liquid crystalline nano-particles (LCNP) have been investigated by cryogenic transmission electron microscopy (cryo-TEM) and synchrotron small angle X-ray diffraction (SAXD). Here we used highly structured cubic micellar (Fd3m) nanoparticles of 50/50 (wt%/wt%) soy phosphatidyl choline (SPC)/glycerol dioleate (GDO) as substrate. Two types of lipolytic enzymes were used, phospholipase A₂ (PLA₂) that catalyses degradation of the phospholipid component, SPC, and porcine pancreatic triacylglycerol lipase (TGL) that facilitate the hydrolysis of the diglyceride, GDO. Evolution of the structure was found to be very different and linked to specificity of the two types of enzymes. PLA₂, which hydrolyses the lamellar forming component, SPC, induces a reversed micellar lipid phase, while TGL which hydrolysis the reverse phase forming compound, GDO, induces a lamellar phase.

通过低温透射电子显微镜（cryo-TEM）和同步加速器小角X射线衍射（SAXD）研究了不同脂肪分解酶对脂质液晶纳米颗粒（LCNP）结构的影响。在这里，我们使用 50/50 (wt%/wt%) 大豆磷脂酰胆碱 (SPC)/甘油二油酸酯 (GDO) 的高度结构化立方胶束 ( Fd3m)纳米粒子作为基质。使用两种类型的脂肪分解酶：催化磷脂成分SPC降解的磷脂酶A ₂ (PLA ₂ )和促进甘油二酯GDO水解的猪胰三酰甘油脂肪酶(TGL)。人们发现结构的演变非常不同，并且与两种酶的特异性有关。 PLA ₂水解层状形成组分SPC，诱导反向胶束脂质相，而TGL 水解反相形成化合物GDO，诱导层状相。