In spite of the importance of the triglyceride aqueous interface for processes like emulsification, surfactant interactions and lipase activity, relatively little is known about this interface compared to that between alkanes and water. Here, the contact between triolein and water was investigated in terms of water inclusion in the oil phase and orientation of the molecules at the interface. Coarse grained models of triglycerides in contact with water were constructed and correlated with experimental results of the changes in thickness and refractive index, obtained using spectroscopic ellipsometry of spin-coated triolein films. The topography of the layer was revealed by atomic force microscopy. Dry triolein and a triolein sample after equilibration with water were also compared structurally using small-angle X-ray scattering. Additionally, the kinetics of adsorption/activity of three different variants of the Thermomyces lanuginosus lipase (TLL) were investigated. The results show that uptake of water in the triolein phase leads to increase in thickness of the layer. The observed increase of thickness was further enhanced by an active lipase but reduced when an inactive mutant of the enzyme was applied.

尽管甘油三酸酯水界面对于诸如乳化，表面活性剂相互作用和脂肪酶活性的过程很重要，但与烷烃和水之间的界面相比，对该界面的了解相对较少。在此，从油相中的水夹杂物和界面处的分子取向方面研究了三油精与水之间的接触。构建了与水接触的甘油三酸酯的粗粒模型，并将其与使用旋涂的三油精薄膜的椭圆偏振光谱法获得的厚度和折射率变化的实验结果相关联。通过原子力显微镜揭示该层的形貌。还使用小角度X射线散射在结构上比较了干燥的三油精和水平衡后的三油精样品。此外，研究了嗜热霉菌脂肪酶（TLL）。结果表明，在三油精相中吸收水导致该层的厚度增加。观察到的厚度增加通过活性脂肪酶进一步增强，但是当应用酶的无活性突变体时降低。