Here, we used centrifugal precipitation to construct a giant vesicle (GV) encapsulating smaller giant vesicles (GV-in-GV) which comprises a photo-resistant outer GV and a photo-pierceable inner GV; the outer GV contained a fluorescent probe (SYBR Green I) in its inner water pool, and the inner GV contained double-stranded DNA (dsDNA) in its inner water pool. The phospholipid membrane of the inner GV was made photo-pierceable by inclusion of ca. 15 mol% of a caged phospholipid in its membrane. Immediately after exposure of the GV-in-GVs to UV irradiation, strong fluorescence was detected in the inner water pool of the outer GV, indicating that dsDNA had been released from the inner GV and had complexed with the fluorescent probe. These dynamics can be recognized as a macroscopic representation of the molecular level function of a caged compound.

在这里，我们使用离心沉淀法构建了一个大囊泡（GV），该囊泡封装了较小的大囊泡（GV-in-GV），该小囊泡包括一个耐光的外部GV和一个可光穿刺的内部GV。外GV在其内部水池中包含一个荧光探针（SYBR Green I），而内部GV在其内部水池中包含双链DNA（dsDNA）。内部GV的磷脂膜可通过加入ca进行光刺穿。膜中有15 mol％的笼状磷脂。将GV-in-GVs暴露于紫外线照射后，立即在外部GV的内部水池中检测到强荧光，表明dsDNA已从内部GV释放并与荧光探针复合。这些动力学可以看作是笼状化合物分子水平功能的宏观表示。