In vitro membrane damage induced by half-fin anchovy hydrolysates/glucose Maillard reaction products and the effects on oxidative status in vivo,Food & Function

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In vitro membrane damage induced by half-fin anchovy hydrolysates/glucose Maillard reaction products and the effects on oxidative status in vivo
Food & Function ( IF 5.1 ) Pub Date : 2017-12-12 00:00:00 , DOI: 10.1039/c7fo01459g
Ru Song _{1,

2,

3,

4} , Qingqing Shi _{1,

2,

3,

4} , Peiyu Yang _{1,

2,

3,

4} , Rongbian Wei _{2,

3,

4,

5}

Affiliation

Membrane damage via the Maillard reaction products of half-fin anchovy hydrolysates and glucose (designated as HAHp(9.0)-G MRPs) was evaluated using an in vitro Escherichia coli (E. coli) model. The incubation of HAHp(9.0)-G MRPs led to a significant increase in outer and inner membrane permeability. Furthermore, membrane integrity was seriously destroyed, as observed by scanning electron microscopy and indicated by the decreased fluorescence signal of 4′,6-diamidino-2-phenylindole stain. Interestingly, HAHp(9.0)-G MRPs induced significant hydrogen peroxide (H₂O₂) production in E. coli after 3 h of incubation, which contributed to the antibacterial activity of HAHp(9.0)-G MRPs. As one of the reactive oxygen species, excess H₂O₂in vivo might impair the antioxidant balance. Therefore, the oxidative status of healthy mice after short-term intake of HAHp(9.0)-G MRPs was investigated. After the administration of HAHp(9.0)-G MRPs at low dose (0.1 g per kg per day body weight) and high dose (1.0 g per kg per day body weight) for 14 days, the body weight of female mice decreased, and the body weight of male mice increased. However, the administration of HAHp(9.0)-G MRPs did not affect the hepatic antioxidant defense in either female or male mice. Some positive responses, like increased superoxide dismutase (SOD) and glutathione peroxidase (GPx) as well as decreased lipid peroxidation (LPO) content, were detected, especially in liver. Though the decrease in catalase activity indicated that the glycation compounds from HAHp(9.0)-G MRPs might be absorbed in vivo, the lower SOD/GPx ratios of HAHp(9.0)-G MRPs-fed groups than those of the normal groups also suggested that the administration of HAHp(9.0)-G MRPs could reduce LPO stress in mice. Our results suggest that a higher antioxidant status could be generated in the healthy animals after fed with HAHp(9.0)-G MRPs for 14 days.

中文翻译：

半翅an鱼水解产物/葡萄糖美拉德反应产物诱导的体外膜损伤及其对体内氧化状态的影响

膜损伤经由半翅凤尾鱼水解物和葡萄糖的美拉德反应产物（命名为HAHp（9.0）-G读护照）使用评价体外大肠杆菌（大肠杆菌）模型。HAHp（9.0）-G MRPs的孵育导致外膜和内膜的渗透性显着增加。此外，如通过扫描电子显微镜观察到的，膜的完整性被严重破坏，并且由4'，6-二ino基-2-苯基吲哚染色的荧光信号降低表明。有趣的是，HAHp（9.0）-G MRP在大肠杆菌中诱导大量过氧化氢（H ₂ O ₂）产生。孵育3小时后，这有助于HAHp（9.0）-G MRP的抗菌活性。作为活性氧之一，体内过量的H ₂ O ₂可能会损害抗氧化剂的平衡。因此，研究了健康小鼠短期摄入HAHp（9.0）-G MRPs后的氧化状态。低剂量（每天每公斤体重0.1克）和高剂量（每天每公斤体重1.0克）给予HAHp（9.0）-G MRP 14天后，雌性小鼠的体重下降，并且雄性小鼠的体重增加。但是，在雌性或雄性小鼠中，HAHp（9.0）-G MRP的施用均不影响肝脏抗氧化防御能力。检测到一些阳性反应，例如增加的超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）以及降低的脂质过氧化（LPO）含量，尤其是在肝脏中。尽管过氧化氢酶活性的降低表明HAHp（9.0）-G MRPs的糖基化化合物可能在体内被吸收，喂食HAHp（9.0）-G MRPs的组的SOD / GPx比正常组低，这也表明给予HAHp（9.0）-G MRPs可以减轻小鼠的LPO应激。我们的结果表明，用HAHp（9.0）-G MRP喂养14天后，健康动物体内可能会产生更高的抗氧化剂状态。

更新日期：2017-12-12

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