Surface coatings that allow externally controlled interaction with cells are of interested for diverse biomedical applications. We fabricated particle patterns and assessed the interaction of these substrates with endothelial cells and hepatocytes. The particles were turned into subcompartmentalized reactors by immobilizing glucose oxidase loaded liposomes sandwiched between polymer layers. The reactor activities depending on the number of liposome deposition steps were confirmed in solution and on patterned surfaces. Finally, reduced viability of hepatocytes adhering to the reactor patterned surfaces in the presence of glucose was observed due to the local production of hydrogen peroxide. This first report on patterned reactors in combination with cells opens up vast opportunities to assemble interactive nanobiointerfaces.

中文翻译：

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细胞与图案化反应器的相互作用†

允许外部控制与细胞相互作用的表面涂层对于多种生物医学应用是令人感兴趣的。我们制造了颗粒图案，并评估了这些底物与内皮细胞和肝细胞的相互作用。通过固定夹在聚合物层之间的负载葡萄糖氧化酶的脂质体，将颗粒转变为亚区室化反应器。在溶液中和在图案化的表面上确认了取决于脂质体沉积步骤数的反应器活性。最后，由于过氧化氢的局部产生，观察到在葡萄糖存在下粘附于反应器图案表面的肝细胞活力降低。关于图案化反应器与细胞结合的第一份报告为组装交互式纳米生物界面提供了巨大的机会。