Production of a biocompatible hyperpolarized bolus for signal amplification by reversible exchange (SABRE) could open the door to simple clinical diagnosis via magnetic resonance imaging. Essential to successful progression to preclinical/clinical applications is the determination of the toxicology profile of the SABRE reaction mixture. Herein, we exemplify the cytotoxicity of the SABRE approach using in vitro cell assays. We conclude that the main cause of the observed toxicity is due to the SABRE catalyst. We therefore illustrate two catalyst removal methods: one involving deactivation and ion‐exchange chromatography, and the second using biphasic catalysis. These routes produce a bolus suitable for future in vivo study.

中文翻译：

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实现生物相容性 SABRE：体外细胞毒性研究


通过可逆交换（SABRE）生产用于信号放大的生物相容性超极化丸剂可以为通过磁共振成像进行简单的临床诊断打开大门。成功推进临床前/临床应用的关键是确定 SABRE 反应混合物的毒理学特征。在此，我们使用体外细胞测定来举例说明 SABRE 方法的细胞毒性。我们得出的结论是，所观察到的毒性的主要原因是 SABRE 催化剂。因此，我们说明了两种催化剂去除方法：一种涉及失活和离子交换色谱法，第二种使用双相催化。这些途径产生适合未来体内研究的丸剂。