C‐reactive protein (CRP) is one of the crucial biomarkers of inflammation, as well as cardiovascular and cancer diseases. Hence, the development of a sensitive and selective technique for CRP detection is of special importance. One of the possibilities is the application of biosensors containing receptor elements formed of RNA aptamers, as they exhibit high affinity towards C‐reactive protein. Herein, the capability of utilization of thiolated RNA aptamers as sensing layers was verified. The occurrence of aptamer ‐ C‐reactive protein interaction was confirmed by quartz crystal microbalance experiments. The voltammetric measurements performed in the presence of methylene blue redox indicator showed a linear current decrease within 1–100 pmol L⁻¹ range of protein concentration. The interaction between surface‐tethered aptamer and CRP was distinguished with dissociation constant of 25.9 pmol L⁻¹. The developed assay demonstrated a pronounced selectivity to CRP over interfering proteins as well as capability of CRP analysis in serum sample.

中文翻译：

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RNA适体作为识别层用于C反应蛋白的电化学分析

C反应蛋白（CRP）是炎症以及心血管疾病和癌症疾病的重要生物标志物之一。因此，开发用于CRP检测的灵敏且选择性的技术具有特别重要的意义。一种可能性是应用包含由RNA适体形成的受体元件的生物传感器，因为它们对C反应蛋白具有很高的亲和力。在此，验证了利用硫醇化的RNA适体作为传感层的能力。石英晶体微量天平实验证实了适体-C反应蛋白相互作用的发生。在亚甲基蓝氧化还原指示剂的存在下进行的伏安测量显示线性电流在1–100 pmol L ^-1之内下降蛋白质浓度范围。表面束缚适体与CRP之间的相互作用具有25.9 pmol L ^-1的解离常数。所开发的测定方法对干扰蛋白具有显着的CRP选择性，并且在血清样品中具有CRP分析能力。