Reagentless Microsensor Based on Conducting Poly(3-aminophenylboronic Acid) for Rapid Detection of Microorganisms in Aerosol,Electroanalysis

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Reagentless Microsensor Based on Conducting Poly(3-aminophenylboronic Acid) for Rapid Detection of Microorganisms in Aerosol
Electroanalysis ( IF 2.7 ) Pub Date : 2017-12-12 , DOI: 10.1002/elan.201700664
Egor A. Andreev ₁ , Maria A. Komkova ₁ , Vera A. Shavokshina ₁ , Denis E. Presnov ₂ , Vladimir A. Krupenin ₂ , Arkady A. Karyakin ₁

Affiliation

We report on microsensor for direct detection of microorganisms in aerosol. Microsensor is based on interdigitated ultramicroelectrodes modified with electropolymerized 3-aminophenylboronic acid (3-APBA). Appearance of Penicillium chrysogenum in aerosol as well as further increase of its concentration lead to poly(3APBA) conductivity increase found by impedance spectroscopy. According to Raman spectroscopy data, the presence of microorganism affects polymer conductivity similarly to binding of glucose to polymer which results in self-doping phenomenon. The elaborated detection technique is 100 times faster comparing to agar plate cultivation: it requires less than 20 minutes for analysis. Dynamic range of microsensor includes the upper limit for non-contaminated air in Russian hygienic standard (500 colony-forming units per cubic meter). The first reported reagentless detection of Penicillium chrysogenum in aerosol by conductivity increase reveals a prospect for creation of a promising alternative to conventional techniques for detection of microorganisms.

中文翻译：

基于导电聚（3-氨基苯基硼酸）的无试剂微传感器快速检测气溶胶中的微生物

我们报告了用于直接检测气溶胶中微生物的微传感器。微传感器基于用电聚合 3-氨基苯基硼酸 (3-APBA) 修饰的叉指式超微电极。Penicillium chrysogenum 在气溶胶中的出现以及其浓度的进一步增加导致了阻抗谱发现的聚（3APBA）电导率增加。根据拉曼光谱数据，微生物的存在会影响聚合物的电导率，类似于葡萄糖与聚合物的结合导致自掺杂现象。与琼脂平板培养相比，精心设计的检测技术要快 100 倍：分析所需的时间不到 20 分钟。微传感器的动态范围包括俄罗斯卫生标准中非污染空气的上限（每立方米500个菌落形成单位）。

更新日期：2017-12-12

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