A graphene oxide (GO) nanosheet-modified N+-nylon membrane (GOM) has been prepared and used as an extraction and spray-ionization substrate for robust mass spectrometric detection of malachite green (MG), a highly toxic disinfectant in liquid samples and fish meat. The GOM is prepared by self-deposition of GO thin film onto an N+-nylon membrane, which has been used for efficient extraction of MG in aquaculture water samples or homogenized fish meat samples. Having a dissociation constant of 2.17 × 10-9 M-1, the GOM allows extraction of approximately 98% of 100 nM MG. Coupling of the GOM-spray with an ion-trap mass spectrometer allows quantitation of MG in aquaculture freshwater and seawater samples down to nanomolar levels. Furthermore, the system possesses high selectivity and sensitivity for the quantitation of MG and its metabolite (leucomalachite green) in fish meat samples. With easy extraction and efficient spray ionization properties of GOM, this membrane spray-mass spectrometry technique is relatively simple and fast in comparison to the traditional LC-MS/MS methods for the quantitation of MG and its metabolite in aquaculture products.

中文翻译：

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氧化石墨烯膜作为高效提取和电离底物，用于鱼类样品中孔雀石绿及其代谢物的喷雾质谱分析

已经制备了氧化石墨烯 (GO) 纳米片改性的 N+-尼龙膜 (GOM)，并将其用作提取和喷雾电离基质，用于对孔雀石绿 (MG) 进行稳健的质谱检测，孔雀石绿 (MG) 是一种液体样品和鱼类中的剧毒消毒剂肉。GOM 是通过将 GO 薄膜自沉积到 N+-尼龙膜上制备的，该膜已用于有效提取水产养殖水样或均质鱼肉样品中的 MG。GOM 的解离常数为 2.17 × 10-9 M-1，可提取约 98% 的 100 nM MG。GOM-spray 与离子阱质谱仪的耦合允许在水产养殖淡水和海水样品中定量低至纳摩尔水平的 MG。此外，该系统对鱼肉样品中 MG 及其代谢物（隐色孔雀石绿）的定量具有高选择性和灵敏度。与传统的 LC-MS/MS 方法相比，GOM 具有易于提取和高效喷雾电离的特性，与用于水产养殖产品中 MG 及其代谢物定量的传统 LC-MS/MS 方法相比，这种膜喷雾质谱技术相对简单和快速。