Homogeneous liquid–liquid extraction (h-LLE) has been receiving considerable attention as a sample preparation method due to its simple and fast partition of compounds with a wide range of polarities. To better understand the differences between the two h-LLE extraction approaches, salting-out assisted liquid–liquid extraction (SALLE) and sugaring-out assisted liquid–liquid extraction (SULLE), have been compared for the partition of 10-hydroxy-2-decenoic acid (10-HDA) from royal jelly, and for the co-extraction of proteins. Effects of the amount of phase partition agents and the concentration of acetonitrile (ACN) on the h-LLE were discussed. Results showed that partition efficiency of 10-HDA depends on the phase ratio in both SALLE and SULLE. Though the partition triggered by NaCl and glucose is less efficient than MgSO₄ in the 50% (v/v) ACN-water mixture, their extraction yields can be improved to be similar with that in MgSO₄ SALLE by increasing the initial concentration of ACN in the ACN-water mixture. The content of co-extracted protein was correlated with water concentration in the obtained upper phase. MgSO₄ showed the largest protein co-extraction at the low concentration of salt. Glucose exhibited a large protein co-extraction in the high phase ratio condition. Furthermore, NaCl with high initial ACN concentration is recommended because it produced high extraction yield for 10-HDA and the lowest amount of co-extracted protein. These observations would be valuable for the sample preparation of royal jelly.

均相液-液萃取（h-LLE）作为一种样品前处理方法已受到广泛关注，因为它可以快速简便地分离极性范围广泛的化合物。为了更好地理解两种h-LLE萃取方法之间的差异，比较了盐析辅助液-液萃取（SALLE）和糖析辅助液-液萃取（SULLE）的10-hydroxy-2分配蜂王浆中的β-癸烯酸（10-HDA），用于蛋白质的共提取。讨论了相分配剂的量和乙腈（ACN）浓度对h-LLE的影响。结果表明10-HDA的分配效率取决于SALLE和SULLE中的相位比。尽管由NaCl和葡萄糖触发的分配效率不如MgSO ₄在50％（v / v）的ACN-水混合物中，通过增加ACN-水混合物中ACN的初始浓度，可以提高其提取率，使其与MgSO ₄ SALLE相似。共提取蛋白的含量与所得上相中的水浓度相关。在低盐浓度下，MgSO ₄表现出最大的蛋白质共萃取。葡萄糖在高相比条件下表现出大量的蛋白质共提取。此外，推荐使用具有较高初始ACN浓度的NaCl，因为它可产生10-HDA的高提取率，并且共提取蛋白的量最少。这些观察对于蜂王浆的样品制备将是有价值的。