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A gold immunochromatographic assay for simultaneous detection of parathion and triazophos in agricultural products†
Analytical Methods ( IF 2.7 ) Pub Date : 2017-12-11 00:00:00 , DOI: 10.1039/c7ay02481a Beibei Liu 1, 2, 3, 4, 5 , Hang Gong 1, 2, 3, 4, 5 , Yulong Wang 1, 2, 3, 4, 5 , Xiaoshuai Zhang 6, 7, 8, 9 , Pan Li 1, 2, 3, 4, 5 , Yulou Qiu 1, 2, 3, 4, 5 , Limin Wang 9, 10, 11, 12 , Xiude Hua 9, 10, 11, 12 , Yirong Guo 9, 13, 14, 15 , Minghua Wang 9, 10, 11, 12 , Fengquan Liu 9, 10, 11, 12 , Xianjin Liu 1, 2, 3, 4, 5 , Cunzheng Zhang 1, 2, 3, 4, 5
Analytical Methods ( IF 2.7 ) Pub Date : 2017-12-11 00:00:00 , DOI: 10.1039/c7ay02481a Beibei Liu 1, 2, 3, 4, 5 , Hang Gong 1, 2, 3, 4, 5 , Yulong Wang 1, 2, 3, 4, 5 , Xiaoshuai Zhang 6, 7, 8, 9 , Pan Li 1, 2, 3, 4, 5 , Yulou Qiu 1, 2, 3, 4, 5 , Limin Wang 9, 10, 11, 12 , Xiude Hua 9, 10, 11, 12 , Yirong Guo 9, 13, 14, 15 , Minghua Wang 9, 10, 11, 12 , Fengquan Liu 9, 10, 11, 12 , Xianjin Liu 1, 2, 3, 4, 5 , Cunzheng Zhang 1, 2, 3, 4, 5
Affiliation
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A multi-component gold immunochromatographic assay (GICA) was developed for the rapid and simultaneous detection of parathion and triazophos in agricultural products. Two different antigens were immobilized separately in two test lines on a nitrocellulose membrane, and two specific monoclonal antibodies (McAbs) against parathion and triazophos were labeled with colloidal gold nanoparticles (AuNPs) as detection probes, respectively. After optimization, the visual cut-off limits of detection for the strip test were 0.05 μg mL−1 for triazophos and 0.1 μg mL−1 for parathion. Thereafter, the developed GICA strip was employed and evaluated for the real sample test using spiked agricultural products (rice, vegetable and fruit samples). The results showed that matrix interference was negligible and the qualification was in good agreement with the results of gas chromatography (GC). The rapid, simple and low cost multidetection sensor could be a potential alternative format for on-site and rapid simultaneous detection of parathion and triazophos.
中文翻译：
同时检测农产品中对硫磷和三唑磷的金免疫色谱法†
开发了一种多组分金免疫色谱分析法（GICA），用于快速，同时检测农产品中的对硫磷和三唑磷。将两种不同的抗原分别固定在硝酸纤维素膜上的两条测试线中，分别用胶体金纳米颗粒（AuNPs）标记两种抗对硫磷和三唑磷的特异性单克隆抗体（McAbs）作为检测探针。优化后，试纸测试的目测截止限为三唑磷为0.05μgmL -1和0.1μgmL -1对硫磷。此后，使用已开发的GICA试纸条，并使用加标的农产品（大米，蔬菜和水果样品）对真实样品进行评估。结果表明，基质干扰可忽略不计，鉴定结果与气相色谱（GC）结果吻合良好。快速，简单和低成本的多重检测传感器可能是对硫磷和三唑磷现场和快速同时检测的潜在替代形式。
更新日期：2017-12-11
中文翻译：
同时检测农产品中对硫磷和三唑磷的金免疫色谱法†
开发了一种多组分金免疫色谱分析法（GICA），用于快速，同时检测农产品中的对硫磷和三唑磷。将两种不同的抗原分别固定在硝酸纤维素膜上的两条测试线中，分别用胶体金纳米颗粒（AuNPs）标记两种抗对硫磷和三唑磷的特异性单克隆抗体（McAbs）作为检测探针。优化后，试纸测试的目测截止限为三唑磷为0.05μgmL -1和0.1μgmL -1对硫磷。此后，使用已开发的GICA试纸条，并使用加标的农产品（大米，蔬菜和水果样品）对真实样品进行评估。结果表明，基质干扰可忽略不计，鉴定结果与气相色谱（GC）结果吻合良好。快速，简单和低成本的多重检测传感器可能是对硫磷和三唑磷现场和快速同时检测的潜在替代形式。
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