Enhanced gene transfection efficiency by use of peptide vectors containing laminin receptor-targeting sequence YIGSR†,Nanoscale

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Enhanced gene transfection efficiency by use of peptide vectors containing laminin receptor-targeting sequence YIGSR†
Nanoscale ( IF 5.8 ) Pub Date : 2017-12-12 00:00:00 , DOI: 10.1039/c7nr05843h
Zhao Meng _{1,

2,

3,

4} , Ziyao Kang _{1,

2,

3,

4} , Chao Sun _{1,

2,

3,

4} , Sen Yang _{1,

2,

3,

4} , Baoquan Zhao _{1,

2,

3,

4} , Siliang Feng _{1,

2,

3,

4} , Qingbin Meng _{1,

2,

3,

4} , Keliang Liu _{1,

2,

3,

4}

Affiliation

This study presents the design and evaluation of a series of multifunctional peptides and their gene delivery abilities. The peptide sequences contained a cell-penetrating segment, six continuous histidine residues, a stearyl moiety and a laminin receptor-targeting segment. The YIGSR segment promoted cellular uptake through the interaction with laminin receptors on the surface of cells, which resulted in a great improvement in gene transfection efficiency. The conformation, particle size and zeta potential of peptide/DNA complexes were characterized via circular dichroism and dynamic light scattering. Their gene transfection efficiency was investigated by fluorescence-activated cell sorting and confocal microscopy. The transfection efficiency of the designed peptide vectors was higher than that of Lipo 2000. The peptide TAT-H₆-K(C₁₈)-YIGSR displayed transfection efficiencies at N/P ratios of 6, which was 3.5 and 7 times higher than that of Lipo 2000 in B16F10 and 293T cells, respectively. All peptides exhibited lower cytotoxicity than Lipo 2000 in B16F10 and 293T cells. In summary, the designed YIGSR-containing multifunctional peptide gene vectors promoted cellular uptake and gene transfection. Their in vivo transfection ability was investigated in zebrafish, and the transfection efficiency was determined by confocal microscopy and bioluminescence imaging. The peptide vectors, owing to their relatively short sequences and ease of functionalization, offer a promising approach for gene delivery because of their low cytotoxicity and high transfection efficiency.

中文翻译：

通过使用含有层粘连蛋白受体靶向序列YIGSR肽载体的增强基因转染效率†

这项研究提出了一系列多功能肽的设计和评估及其基因传递能力。肽序列包含细胞穿透区段，六个连续的组氨酸残基，硬脂基部分和层粘连蛋白受体靶向区段。YIGSR区段通过与细胞表面层粘连蛋白受体的相互作用促进细胞摄取，从而大大提高了基因转染效率。肽/ DNA复合物的构象，粒径和ζ电势通过圆二色性和动态光散射。通过荧光激活细胞分选和共聚焦显微镜研究了它们的基因转染效率。设计的肽载体的转染效率高于Lipo2000 。肽TAT-H ₆ -K（C ₁₈）-YIGSR在N / P比为6时显示出转染效率，分别为Lipo 2000和Lipo 2000的3.5和7倍。 Lipo 2000分别在B16F10和293T细胞中的表达。在B16F10和293T细胞中，所有肽均显示出比Lipo 2000低的细胞毒性。总之，设计的含YIGSR的多功能肽基因载体可促进细胞摄取和基因转染。他们的体内研究了斑马鱼的转染能力，并通过共聚焦显微镜和生物发光成像确定了转染效率。肽载体由于其相对短的序列和容易的功能化，由于其低细胞毒性和高转染效率而为基因递送提供了有希望的方法。

更新日期：2017-12-12

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