Production, optimisation and characterisation of angiotensin converting enzyme inhibitory peptides from sea cucumber (Stichopus japonicus) gonad†,Food & Function

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Production, optimisation and characterisation of angiotensin converting enzyme inhibitory peptides from sea cucumber (Stichopus japonicus) gonad†
Food & Function ( IF 5.1 ) Pub Date : 2017-12-11 00:00:00 , DOI: 10.1039/c7fo01388d
Chan Zhong _{1,

2,

3,

4} , Le-Chang Sun _{1,

2,

3,

4,

5} , Long-Jie Yan _{1,

2,

3,

4} , Yi-Chen Lin _{1,

2,

3,

4} , Guang-Ming Liu _{1,

2,

3,

4,

5} , Min-Jie Cao _{1,

2,

3,

4,

5}

Affiliation

In this study, production of bioactive peptides with angiotensin converting enzyme (ACE) inhibitory activity from sea cucumber (Stichopus japonicus) gonad using commercial protamex was optimised by response surface methodology (RSM). As a result, the optimal condition to achieve the highest ACE inhibitory activity in sea cucumber gonad hydrolysate (SCGH) was hydrolysis for 1.95 h and E/S of 0.75%. For further characterisation, three individual peptides (EIYR, LF and NAPHMR) were purified and identified. The peptide NAPHMR showed the highest ACE inhibitory activity with IC₅₀ of 260.22 ± 3.71 μM. NAPHMR was stable against simulated gastrointestinal digestion and revealed no significant cytotoxicity toward Caco-2 cells. Molecular docking study suggested that Arg, His and Asn residues in NAPHMR interact with the S2 pocket or Zn²⁺ binding motifs of ACE via hydrogen or π-bonds, potentially contributing to ACE inhibitory effect. Sea cucumber gonad is thus a potential resource to produce ACE inhibitory peptides for preparation of functional foods.

中文翻译：

生产，优化和海参转化酶抑制肽血管紧张素表征（刺参）性腺†

在这项研究中，通过响应面方法（RSM）优化了使用商业Protamex从海参（Stichopus japonicus）性腺中产生具有血管紧张素转化酶（ACE）抑制活性的生物活性肽。结果，在海参生殖腺水解产物（SCGH）中获得最高ACE抑制活性的最佳条件是水解1.95小时，E / S为0.75％。为了进一步表征，纯化并鉴定了三种单独的肽（EIYR，LF和NAPHMR）。肽NAPHMR在IC _50下显示出最高的ACE抑制活性260.22±3.71微米 NAPHMR对模拟的消化道消化是稳定的，对Caco-2细胞没有明显的细胞毒性。分子对接研究表明，NAPHMR中的Arg，His和Asn残基通过氢或π键与ACE的S2口袋或Zn ²⁺结合基序相互作用，可能有助于ACE抑制作用。因此，海参性腺是生产ACE抑制肽以制备功能性食品的潜在资源。

更新日期：2017-12-11

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