Fresh collections, type studies and molecular phylogenetic analyses of a multigene matrix of partial nuSSU-ITS-LSU rDNA, rpb2, tef1 and tub2 sequences were used to evaluate the boundaries of Cucurbitaria in a strict sense and of several related genera of the Cucurbitariaceae. Two species are recognised in Cucurbitaria and 19 in Neocucurbitaria. The monotypic genera Astragalicola, Cucitella, Parafenestella, Protofenestella, and Seltsamia are described as new. Fenestella is here included as its generic type F. fenestrata (= F. princeps), which is lecto- and epitypified. Fenestella mackenzei and F. ostryae are combined in Parafenestella. Asexual morphs of Cucurbitariaceae, where known, are all pyrenochaeta- or phoma-like. Comparison of the phylogenetic analyses of the ITS-LSU and combined matrices demonstrate that at least rpb2 sequences should be added whenever possible to improve phylogenetic resolution of the tree backbone; in addition, the tef1 introns should be added as well to improve delimitation of closely related species.

新鲜的集合，类型研究和局部nuSSU-ITS-LSU rDNA的，多基因矩阵的分子系统发生分析RPB2，TEF1和TUB2序列用于评估的边界Cucurbitaria在严格意义上和几个相关属的Cucurbitariaceae。南瓜属中确认有2种，新南瓜属中确认了19种。单属的黄芪，Cucitella，Parafenestella，Protofenestella和Seltsamia属被描述为新的。费内斯特拉此处包含F. fenestrata（= F. princeps）作为其通用类型，这是经选集和表型化的。Fenestella mackenzei和F. ostryae合并在Parafenestella中。葫芦科的无性变体，全都像变色龙或类似phoma。对ITS-LSU和组合矩阵的系统发育分析的比较表明，应尽可能增加至少rpb2序列，以提高树主干的系统发育分辨率。此外，还应添加tef1内含子，以改善紧密相关物种的定界。