Realization of a stable, monodisperse water-in-oil droplet system with micro-scale and nano-scale confinement for tandem microscopy and diffusion NMR studies,Soft Matter

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Realization of a stable, monodisperse water-in-oil droplet system with micro-scale and nano-scale confinement for tandem microscopy and diffusion NMR studies
Soft Matter ( IF 2.9 ) Pub Date : 2017-12-11 00:00:00 , DOI: 10.1039/c7sm01508a
Swomitra Palit _{1,

2,

3,

4} , Somayeh Khajehpour Tadavani _{1,

2,

3,

4} , Anand Yethiraj _{1,

2,

3,

4}

Affiliation

In this work we generate stable and monodisperse water-in-oil emulsions using a co-flowing geometry that produced droplet sizes between 13 μm and 250 μm. The drops survived transfer to NMR tubes and were stable for at least 26 hours, enabling the performance of pulsed-field-gradient NMR experiments in addition to microscopy. The drops sizes achieved as a function of flow rate agree well with a simple model for droplet generation: this yields a precise measure of the interfacial tension. The design of a cell mimetic environment with nano-scale confinement has also been demonstrated with diffusion measurements on macromolecules (PEG and Ficoll70) within droplets that are further structured internally using agarose gel networks. Containing the agarose gel in droplets appears to provide very reproducible and homogeneous network environments, enabling quantitative agreement of Ficoll70 dynamics with a theoretical model, with no fit parameters, and, with PEG, yielding a systematic polymer-size dependent slowing down in the network. This is in contrast with bulk agarose, where identical macromolecular diffusion measurements indicate the presence of heterogeneities with water pockets.

中文翻译：

实现了用于串联显微镜和扩散NMR研究的具有微米级和纳米级限制的稳定的单分散油包水液滴系统

在这项工作中，我们使用共同流动的几何形状产生稳定且单分散的油包水型乳液，产生的液滴尺寸在13μm至250μm之间。液滴在转移到NMR管中幸存下来，并且稳定至少26小时，因此除了显微镜检查外，还可以执行脉冲场梯度NMR实验。作为流量函数的液滴尺寸与液滴产生的简单模型非常吻合：这可以精确测量界面张力。还通过对大分子（PEG和Ficoll70）在液滴中的扩散测量进行了纳米级限制的模拟细胞环境的设计，该液滴使用琼脂糖凝胶网络在内部进行了进一步结构化。液滴中含有琼脂糖凝胶似乎可以提供非常可重现且均质的网络环境，使用理论模型（没有合适的参数）使Ficoll70动力学定量吻合，并且使用PEG，可以根据系统的聚合物大小进行减慢，从而降低网络速度。这与大量琼脂糖形成对照，在琼脂糖中相同的大分子扩散测量表明存在水袋的异质性。

更新日期：2017-12-11

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