Inflammation is the initial phase in the healing of cutaneous wounds; however, persistent inflammation will hamper the healing process by generating excess inflammatory cytokines and reactive oxygen species (ROS). Therefore, preventing persistent inflammation and clearing redundant ROS are important strategies in accelerating wound healing. Owing to their unique redox activity, cerium oxide (CeO₂) nanoparticles have shown promising potential as antioxidative and anti-inflammatory agents for the treatment of various diseases resulting from oxidative stress. In the present study, we prepared chitosan-coated CeO₂ nanocubes (CCNs) and evaluated their cutaneous wound healing potential when topically applied to open excision wounds on adult Sprague Dawley (SD) rats. CCN application significantly increased the wound healing rates and showed superior wound healing capabilities compared to a clinically applied wound healing agent, recombinant human epidermal growth factor (rhEGF). We attribute this superior wound healing ability to their anti-inflammatory ability by decreasing the expression of the inflammatory cytokine tumor necrosis factor-alpha (TNF-α) and increasing the expression of the anti-inflammatory cytokine interleukin-10 (IL-10), as well as to their antioxidative ability by increasing antioxidant enzyme levels. These results suggest that CCNs hold therapeutic potential in treating refractory wounds characterized by persistent inflammation caused by oxidative-stress related diseases such as diabetes.

中文翻译：

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壳聚糖涂层的氧化铈纳米立方体可通过减少持续性炎症来加速皮肤伤口愈合†

炎症是皮肤伤口愈合的初始阶段。但是，持续的炎症会通过产生过量的炎症细胞因子和活性氧（ROS）来阻碍愈合过程。因此，预防持续性炎症和清除多余的ROS是加速伤口愈合的重要策略。由于其独特的氧化还原活性，氧化铈（CeO ₂）纳米颗粒已显示出作为抗氧化剂和抗炎剂的潜力，可用于治疗由氧化应激引起的各种疾病。在本研究中，我们制备了壳聚糖包覆的CeO ₂纳米立方（CCNs）并评估了它们在成年Sprague Dawley（SD）大鼠的开放性切开伤口上局部使用时的皮肤伤口愈合潜力。与临床应用的伤口愈合剂，重组人表皮生长因子（rhEGF）相比，CCN的应用显着提高了伤口的愈合速度，并显示出优异的伤口愈合能力。我们通过降低炎症细胞因子肿瘤坏死因子-α（TNF-α）的表达并增加抗炎症细胞因子白介素10（IL-10）的表达，将这种优异的伤口愈合能力归因于其抗炎能力，以及通过增加抗氧化酶水平来提高其抗氧化能力。