Chitosan/PEI patch releasing EGF and the EGFR gene for the regeneration of the tympanic membrane after perforation†,Biomaterials Science

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Chitosan/PEI patch releasing EGF and the EGFR gene for the regeneration of the tympanic membrane after perforation†
Biomaterials Science ( IF 5.8 ) Pub Date : 2017-12-08 00:00:00 , DOI: 10.1039/c7bm01061c
Myung Chul Lee _{1,

2,

3,

4} , Hoon Seonwoo _{4,

5,

6,

7} , Pankaj Garg _{2,

4,

8,

9} , Kyoung Je Jang _{1,

2,

3,

4} , Shambhavi Pandey _{2,

4,

8,

9} , Sang Bae Park _{1,

2,

3,

4} , Hong Bae Kim _{1,

2,

3,

4} , Jaewoon Lim _{1,

2,

3,

4} , Yun Hoon Choung _{4,

10,

11,

12} , Jong Hoon Chung _{1,

2,

3,

4,

8}

Affiliation

Damage to the eardrum causes acute pain and can lead to chronic otitis media if it develops into chronic tympanic membrane (TM) perforations. Chronic TM perforations are usually treated with surgical methods such as tympanoplasty and myringoplasty. However, these surgeries are not only complicated and difficult but also cost a lot of money. Our research team developed chitosan patches (E-CPs) that release epidermal growth factor (EGF) as a patch therapy to replace surgical methods. However, there was a limitation in the healing ratio of the treatment compared to the surgical methods. In this study, we developed EGF and epidermal growth factor receptor (EGFR) gene-releasing polyethyleneimine (PEI)/chitosan patches (EErP-CPs) to increase the regeneration of TM perforations. The addition of PEI increased the adhesion and migration ability of TM cells on the patches. The simultaneous release of the EGF and the EGFR gene further enhanced TM cell proliferation, adhesion and migratory ability. It was confirmed that the EGF protein and EGFR gene were released for 30 days; however, EGF was released and increased TM cell viability almost immediately after treatment and EGFR took a minimum of 3 days before showing its effect on improved cell viability. It was also shown that EErP-CPs are more hydrophilic and have more positive charge than E-CP because of added amine groups from PEI. In conclusion, the developed EErP-CPs resulted in the improved healing of TM perforations and can potentially be applied to the regeneration of both chronic and acute tympanic membrane perforations.

中文翻译：

壳聚糖/ PEI贴片释放EGF和EGFR基因，用于穿孔后鼓膜的再生†

鼓膜损伤会引起急性疼痛，如果发展成慢性鼓膜（TM）穿孔，则可能导致慢性中耳炎。慢性TM穿孔通常采用鼓膜成形术和鼓膜成形术等手术方法进行治疗。但是，这些手术不仅复杂且困难，而且花费很多钱。我们的研究小组开发了脱乙酰壳多糖贴剂（E-CP），可释放表皮生长因子（EGF）作为贴剂疗法来替代外科手术方法。但是，与手术方法相比，治疗的治愈率存在局限性。在这项研究中，我们开发了EGF和释放表皮生长因子受体（EGFR）基因的聚乙烯亚胺（PEI）/壳聚糖贴片（EErP-CPs），以增加TM穿孔的再生。PEI的添加增加了TM细胞在贴片上的粘附和迁移能力。EGF和EGFR基因的同时释放进一步增强了TM细胞的增殖，粘附和迁移能力。证实EGF蛋白和EGFR基因被释放了30天。然而，治疗后几乎立即释放了EGF，并增加了TM细胞的活力，而EGFR至少花了3天才显示出它对改善细胞活力的作用。还显示出EErP-CP比E-CP更亲水并且具有更多的正电荷，这是由于PEI中添加了胺基。总之，发达的EErP-CPs可以改善TM穿孔的愈合，并且可以潜在地应用于慢性和急性鼓膜穿孔的再生。EGF和EGFR基因的同时释放进一步增强了TM细胞的增殖，粘附和迁移能力。证实EGF蛋白和EGFR基因被释放了30天。然而，治疗后几乎立即释放了EGF，并增加了TM细胞的活力，而EGFR至少花了3天才显示出它对改善细胞活力的作用。还显示出EErP-CP比E-CP更亲水并且具有更多的正电荷，这是由于PEI中添加了胺基。总之，发达的EErP-CPs可以改善TM穿孔的愈合，并且可以潜在地应用于慢性和急性鼓膜穿孔的再生。EGF和EGFR基因的同时释放进一步增强了TM细胞的增殖，粘附和迁移能力。证实EGF蛋白和EGFR基因被释放了30天。然而，治疗后几乎立即释放了EGF，并增加了TM细胞的活力，而EGFR至少花了3天才显示出它对改善细胞活力的作用。还显示出EErP-CP比E-CP更亲水并且具有更多的正电荷，这是由于PEI中添加了胺基。总之，发达的EErP-CPs可以改善TM穿孔的愈合，并且可以潜在地应用于慢性和急性鼓膜穿孔的再生。证实EGF蛋白和EGFR基因被释放了30天。然而，治疗后几乎立即释放了EGF，并增加了TM细胞的活力，而EGFR至少花了3天才显示出它对改善细胞活力的作用。还显示出EErP-CP比E-CP更亲水并且具有更多的正电荷，这是由于PEI中添加了胺基。总之，发达的EErP-CPs可以改善TM穿孔的愈合，并且可以潜在地应用于慢性和急性鼓膜穿孔的再生。证实EGF蛋白和EGFR基因被释放了30天。然而，治疗后几乎立即释放了EGF，并增加了TM细胞的活力，而EGFR至少花了3天才显示出它对改善细胞活力的作用。还显示出EErP-CP比E-CP更亲水并且具有更多的正电荷，这是由于PEI中添加了胺基。总之，已开发的EErP-CP可以改善TM穿孔的愈合，并且可以潜在地应用于慢性和急性鼓膜穿孔的再生。还显示出EErP-CP比E-CP更亲水并且具有更多的正电荷，这是由于PEI中添加了胺基。总之，发达的EErP-CPs可以改善TM穿孔的愈合，并且可以潜在地应用于慢性和急性鼓膜穿孔的再生。还显示出EErP-CP比E-CP更亲水并且具有更多的正电荷，这是由于PEI中添加了胺基。总之，发达的EErP-CPs可以改善TM穿孔的愈合，并且可以潜在地应用于慢性和急性鼓膜穿孔的再生。

更新日期：2017-12-08

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