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Circulation Research ( IF 16.5 ) Pub Date : 2017-12-08 , DOI: 10.1161/res.0000000000000187
Ruth Williams

### Parikh et al develop a protocol for promoting t-tubule formation in human iPSC-derived cardiomyocytes. Cardiac myocytes derived from human induced pluripotent stem cells (hiPSC-CMs) are useful for modeling a variety of heart diseases and potentially for repairing injured hearts. But the process of in vitro hiPSC-CM differentiation generally fails to produce fully mature cells. For example, they tend to lack t-tubules—membranous invaginations that transverse the cell and are essential for normal cellular electrophysiology. Previous strategies for improving hiPSC-CM maturation include growing the cells on a matrix of physiological stiffness (matrigel mattress) and adding thyroid and glucocorticoid hormones to the media. However, neither of these approaches promote fully fledged t-tubule development. Parikh and colleagues have now discovered that by …

中文翻译:

在这个问题上

### Parikh等人开发了一种在人iPSC衍生的心肌细胞中促进t小管形成的方案。源自人诱导性多能干细胞(hiPSC-CM)的心肌细胞可用于建模各种心脏病,并可能用于修复受伤的心脏。但是体外hiPSC-CM分化过程通常无法产生完全成熟的细胞。例如,它们倾向于缺乏t型小管,即跨细胞的膜状内陷,对于正常的细胞电生理是必不可少的。改善hiPSC-CM成熟的先前策略包括在具有生理刚度的基质(基质胶床垫)上生长细胞,并向培养基中添加甲状腺激素和糖皮质激素。但是,这两种方法均不能促进成熟的T形管发育。
更新日期:2017-12-07
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