### Parikh et al develop a protocol for promoting t-tubule formation in human iPSC-derived cardiomyocytes. Cardiac myocytes derived from human induced pluripotent stem cells (hiPSC-CMs) are useful for modeling a variety of heart diseases and potentially for repairing injured hearts. But the process of in vitro hiPSC-CM differentiation generally fails to produce fully mature cells. For example, they tend to lack t-tubules—membranous invaginations that transverse the cell and are essential for normal cellular electrophysiology. Previous strategies for improving hiPSC-CM maturation include growing the cells on a matrix of physiological stiffness (matrigel mattress) and adding thyroid and glucocorticoid hormones to the media. However, neither of these approaches promote fully fledged t-tubule development. Parikh and colleagues have now discovered that by …

中文翻译：

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### Parikh等人开发了一种在人iPSC衍生的心肌细胞中促进t小管形成的方案。源自人诱导性多能干细胞（hiPSC-CM）的心肌细胞可用于建模各种心脏病，并可能用于修复受伤的心脏。但是体外hiPSC-CM分化过程通常无法产生完全成熟的细胞。例如，它们倾向于缺乏t型小管，即跨细胞的膜状内陷，对于正常的细胞电生理是必不可少的。改善hiPSC-CM成熟的先前策略包括在具有生理刚度的基质（基质胶床垫）上生长细胞，并向培养基中添加甲状腺激素和糖皮质激素。但是，这两种方法均不能促进成熟的T形管发育。