Single molecule spectroscopy has undergone a remarkable development in the past few decades, and its ability to unmask the unique features of individual molecules has found increasing use in research of soft matter and polymers, in chemistry, as well as in biophysics and biology. In this concise review we bring an overview of the synergy effects that result from combinations of single-molecule and single-particle fluorescence spectroscopy with other techniques, such as electron microscopy and scanning probe microscopy, or with external-field effects including hydrostatic pressure and electric and magnetic fields.

中文翻译：

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光学成像之外的单分子研究：多参数单分子光谱

在过去的几十年中，单分子光谱学取得了举世瞩目的发展，其揭示单个分子独特特征的能力在软物质和聚合物的研究，化学以及生物物理学和生物学中得到了越来越多的应用。在此简明扼要的综述中，我们概述了单分子和单粒子荧光光谱与其他技术（例如电子显微镜和扫描探针显微镜）或外部场效应（包括静水压力和电学效应）的组合所产生的协同效应。和磁场。