The electrochemical activity of electroactive bacteria is unique and essential for bioelectrochemical systems (BES) with promising energy and environment applications. However, the strategies for bacterial electrochemical activity modulation are still limited. Herein, a novel approach for engineering of bacterial electrochemical activity with global regulator (sigma factor RpoS) manipulation was developed. By knockout of rpoS in Pseudomonas aeruginosa PAO1, the biofilm formation on the electrode was enhanced and the bacterial cells were adapted to a new electron shuttle, which promoted the extracellular electron transfer and improved the performance of BES. This work demonstrated that global regulator manipulation could be efficient for electrochemical activity engineering and offered new opportunities for BES applications.

电活性细菌的电化学活性是独特的，对于生物电化学系统（BES）具有广阔的能源和环境应用至关重要。但是，细菌电化学活性调节的策略仍然受到限制。本文中，开发了一种利用全局调节剂（σ因子RpoS）操纵工程化细菌电化学活性的新方法。通过对敲的rpoS在铜绿假单胞菌PAO1增强了电极上的生物膜形成，并使细菌细胞适应了新的电子穿梭，从而促进了细胞外电子的转移并改善了BES的性能。这项工作表明，全局调节器操纵对于电化学活性工程可能是有效的，并为BES应用提供了新的机会。