Our continuous search for new nitric oxide (NO) inhibitory substances as anti-neuroinflammatory agents for AD resulted in the isolation of one new labdane diterpenoid and three new guaiane sesquiterpenoids, as well as ten known compounds from Blumea balsamifera. Their structures were elucidated by NMR spectroscopic data analysis and the time-dependent density functional theory (TDDFT) electronic circular dichroism (ECD) calculations. The anti-neuroinflammatory effects were examined by inhibiting NO release in LPS-induced murine microglial BV-2 cells. The possible mechanism of NO inhibition of some bioactive compounds was also investigated using molecular docking, which revealed the interactions of bioactive compounds with the iNOS protein.

我们不断寻找新的一氧化氮（NO）抑制物质作为AD的抗神经炎药，导致分离出一种新的拉丹烷二萜和三种新的愈创树倍半萜，以及十种从Balmea balsamifera获得的已知化合物。通过NMR光谱数据分析和时间依赖性密度泛函理论（TDDFT）电子圆二色性（ECD）计算，阐明了它们的结构。通过抑制LPS诱导的鼠小神经胶质BV-2细胞中NO的释放来检查抗神经炎作用。还使用分子对接研究了某些生物活性化合物的NO抑制的可能机理，这揭示了生物活性化合物与iNOS蛋白的相互作用。