Sample preparation is an indispensable part of proteomics research. Owing to its merits of large surface area, narrow pore size distribution, adjustable pore size, modifiable pore inwalls and so on, mesoporous materials have been widely developed as one of main techniques for sample preparation in proteomics. The uniform mesopores allow small size peptides into mesoporous channels while large size proteins excluded. In addition, large surface area of mesoporous materials could provide more binding sites for modification, and thus improving selectivity of materials and increasing sensitivity during MS analysis. In this review, recent advances in mesoporous materials as sample preparation technique, including enrichment of endogenous peptides, specific enrichment of phosphopeptides and glycopeptides as well as protein digestion were summarized and discussed comprehensively. Furthermore, concerns in synthesis of mesoporous materials and enrichment biases of different functionalized mesoporous materials towards target peptides as well as the problem of sample analysis are also prospected.

样品制备是蛋白质组学研究必不可少的部分。由于介孔材料具有比表面积大，孔径分布窄，孔​​径可调节，孔壁可调节等优点，因此被广泛开发为蛋白质组学中样品制备的主要技术之一。均匀的中孔允许小尺寸的肽进入中孔通道，而大尺寸的蛋白被排除在外。此外，介孔材料的大表面积可提供更多的结合位点以进行修饰，从而提高了材料的选择性，并提高了MS分析过程中的灵敏度。本文综述了介孔材料作为样品制备技术的最新进展，包括内源肽的富集，总结和讨论了磷酸肽和糖肽的特异性富集以及蛋白质的消化。此外，还预期到介孔材料的合成和不同功能化介孔材料对靶肽的富集偏倚以及样品分析的问题。