Hierarchical “As-Electrospun” Self-Assembled Fibrous Scaffolds Deconvolute Impacts of Chemically Defined Extracellular Matrix- and Cell Adhesion-Type Interactions on Stem Cell Haptokinesis,ACS Macro Letters

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Hierarchical “As-Electrospun” Self-Assembled Fibrous Scaffolds Deconvolute Impacts of Chemically Defined Extracellular Matrix- and Cell Adhesion-Type Interactions on Stem Cell Haptokinesis
ACS Macro Letters ( IF 5.1 ) Pub Date : 2017-12-05 00:00:00 , DOI: 10.1021/acsmacrolett.7b00834
Sandra Camarero-Espinosa ₁ , Ilaria Stefani ₁ , Justin Cooper-White _{1,

2,

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Affiliation

Controlled self-assembly of diblock copolymers offers the possibility of fabricating multilength scale, three-dimensional (3D) porous/fibrous structures (or scaffolds) with defined internal nano- or microstructure, with opportunities for application in a variety of fields, ranging from energy storage to bioengineering. Traditional methods by which such 3D constructs are produced are time-consuming and tedious, hindering their broader exploitation within larger-scale industrial processes. We report the development of a one-step process to fabricate “as-electrospun” self-assembled diblock copolymer micro- to nanometer-sized fibers incorporating core–shell or lamellar, closely packed spheres or bicontinuous gyroid nanosized structures. Isotropic and anisotropic (aligned) porous mats presenting spatially controlled chemistries, including bioactive (peptide-based) motifs, were successfully made from these hierarchical fibers. When functionalized with peptide sequences derived from a cell adhesion molecule (E-cadherin) and an extracellular matrix glycoprotein (laminin), these novel materials provided new insight into the impacts of such exquisitely tailored contact-guidance cues on the haptokinesis of human mesenchymal stem cells.

中文翻译：

分层“电纺”自组装纤维支架解卷积化学定义的细胞外基质和细胞粘附型相互作用对干细胞触觉运动的影响

二嵌段共聚物的受控自组装提供了制造具有确定的内部纳米或微结构的多长度尺度、三维 (3D) 多孔/纤维结构（或支架）的可能性，并有机会应用于各种领域，从能源储存到生物工程。生产此类 3D 构造的传统方法既耗时又乏味，阻碍了它们在更大规模的工业过程中的更广泛开发。我们报告了一种一步法制造“电纺”自组装二嵌段共聚物微米至纳米尺寸纤维的发展，该纤维包含核壳或层状、紧密堆积的球体或双连续螺旋状纳米结构。各向同性和各向异性（对齐）多孔垫呈现空间受控的化学成分，包括生物活性（基于肽的）基序，均由这些分层纤维成功制成。当用源自细胞粘附分子的肽序列进行功能化时（E-钙粘蛋白）和细胞外基质糖蛋白（层粘连蛋白），这些新材料为这种精心定制的接触指导线索对人类间充质干细胞的触觉运动的影响提供了新的见解。

更新日期：2017-12-05

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