The efficiency of gold nanoparticle (AuNP) dimerization mediated by hybridization between two probe DNA molecules and a complementary target DNA molecule was maximized by examining several possible hybridization combinations. The uniformity of the size of the AuNPs, the use of surface modification appropriate for high hybridization efficiency, together with efficient blocking of nonspecific binding, all contributed to achieving a 1 pM detection limit following conventional gel electrophoresis separation of the DNA-modified AuNP multimers. This practical homogeneous DNA hybridization assay methodology will provide a rapid, cost-effective, and field-portable tool for clinical diagnosis.

中文翻译：

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使用金纳米颗粒和电泳分离的DNA杂交测定可提供1 pM的灵敏度

通过检查几种可能的杂交组合，通过两个探针DNA分子与互补靶DNA分子之间的杂交介导的金纳米颗粒（AuNP）二聚化效率达到最大。AuNPs大小的均匀性，适合高杂交效率的表面修饰的使用以及对非特异性结合的有效阻断，都有助于在DNA修饰的AuNP多聚体的常规凝胶电泳分离后达到1 pM的检测限。这种实用的均质DNA杂交测定方法学将为临床诊断提供快速，经济高效且可现场携带的工具。