Access to chromatin for processes such as transcription and DNA repair requires the sliding of nucleosomes along DNA. This process is aided by chromatin-remodeling complexes, such as the multisubunit INO80 chromatin-remodeling complex. Here we present cryo-EM structures of the active core complex of human INO80 at 9.6 Å, with portions at 4.1-Å resolution, and reconstructions of combinations of subunits. Together, these structures reveal the architecture of the INO80 complex, including Ino80 and actin-related proteins, which is assembled around a single RUVBL1 (Tip49a) and RUVBL2 (Tip49b) AAA+ heterohexamer. An unusual spoked-wheel structural domain of the Ino80 subunit is engulfed by this heterohexamer; both, in combination, form the core of the complex. We also identify a cleft in RUVBL1 and RUVBL2, which forms a major interaction site for partner proteins and probably communicates these interactions to its nucleotide-binding sites.

中文翻译：

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人类 INO80 染色质重塑复合物的冷冻电镜结构。

进入染色质进行转录和 DNA 修复等过程需要核小体沿着 DNA 滑动。这一过程得到染色质重塑复合物的帮助，例如多亚基 INO80 染色质重塑复合物。在这里，我们展示了 9.6 Å 的人类 INO80 活性核心复合物的低温 EM 结构，部分分辨率为 4.1 Å，以及亚基组合的重建。总之，这些结构揭示了 INO80 复合物的结构，包括 Ino80 和肌动蛋白相关蛋白，它们围绕单个 RUVBL1 (Tip49a) 和 RUVBL2 (Tip49b) AAA+ 异六聚体组装。Ino80 亚基的一个不寻常的轮辐结构域被这种异六聚体所吞噬；两者结合起来，构成了综合体的核心。我们还发现了 RUVBL1 和 RUVBL2 中的一个裂缝，