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The new 2D Superresolution mode for ZEISS Airyscan
Nature Methods ( IF 36.1 ) Pub Date : 2017-11-30 , DOI: 10.1038/nmeth.f.404
Joseph Huff , Annette Bergter , Jan Birkenbeil , Ingo Kleppe , Ralf Engelmann , Uros Krzic

Utilizing a pinhole-plane imaging concept, Airyscan allows for simultaneous improvement in resolution and signal-to-noise by capitalizing on an innovative 32-channel GaAsP photomultiplier tube (PMT) array detector. Each detection channel functions as a very small pinhole to increase resolution while the overall detector design delivers better signal-to-noise than traditional GaAsP-based confocal systems. In the past, a stack of at least five z-slices had to be deconvolved to get usable images with an optical section thinner than 1 Airy unit. Now, the new 2D Superresolution mode for Airyscan delivers images with the thinnest optical section (0.2 Airy units) from a single image while maintaining the light collection efficiency of a much larger 1.25-Airy-unit pinhole.



中文翻译:

蔡司Airyscan的新2D超分辨率模式

利用针孔平面成像概念,Airyscan通过利用创新的32通道GaAsP光电倍增管(PMT)阵列检测器,可以同时提高分辨率和信噪比。每个检测通道都充当一个很小的针孔,以提高分辨率,而整个检测器设计比传统的基于GaAsP的共聚焦系统可提供更好的信噪比。过去,必须对至少五个z切片的堆栈进行反卷积处理,以获取具有小于1 Airy单位的光学部分的可用图像。现在,用于Airyscan的新的2D超分辨率模式可以从单个图像提供光学截面最薄的图像(0.2 Airy单位),同时保持更大的1.25 Airy单位针孔的光收集效率。

更新日期:2017-12-05
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