Lipase from Mucor miehei was immobilized on chitosan hydrogels without using any activation agent. These low-cost and easy to prepare lipase-containing hydrogels could be considered as novel solid-phase biocatalysts for use in organic media. When they were applied as biocatalysts towards the model esterification reaction of propyl laurate synthesis they showed an optimum operation temperature at 30 °C and maintained a high catalytic activity in various non-polar organic solvents. The higher initial esterification rates were obtained when biocatalysis took place in branched hydrocarbons where the initial rates recorded were twice the ones observed for straight hydrocarbons, being 0.22 mM/min in isooctane and 0.11 mM/min in hexane, respectively. The novel catalyst presented excellent reusability, with only 10% loss of activity after 12 uses, which is even higher than the one obtained by lipase-containing chitosan beads prepared with a cross-linker. Moreover, enzyme superactivity was evident for several runs.

来自Mucor miehei的脂肪酶在不使用任何活化剂的情况下将其固定在壳聚糖水凝胶上。这些低成本且易于制备的含脂肪酶的水凝胶可被视为用于有机介质的新型固相生物催化剂。当将它们用作月桂酸丙酯合成的模型酯化反应的生物催化剂时，它们在30°C时显示最佳操作温度，并在各种非极性有机溶剂中保持较高的催化活性。当在支链烃中进行生物催化时，可获得较高的初始酯化速率，其中记录的初始速率是纯烃观察到的初始速率的两倍，分别为异辛烷中的0.22 mM / min和己烷中的0.11 mM / min。新型催化剂具有出色的可重复使用性，使用12次后活性仅损失10％，它甚至比用交联剂制备的含脂肪酶的壳聚糖珠所获得的珠子还要高。而且，酶的超活性对于数次运行是明显的。