Functional and Proteomic Alterations of F1 Capacitated Spermatozoa of Adult Mice Following Gestational Exposure to Bisphenol A,Journal of Proteome Research

当前位置： X-MOL 学术 › J. Proteome Res. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Functional and Proteomic Alterations of F1 Capacitated Spermatozoa of Adult Mice Following Gestational Exposure to Bisphenol A
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2017-12-21 00:00:00 , DOI: 10.1021/acs.jproteome.7b00668
Md Saidur Rahman ₁ , Woo-Sung Kwon ₁ , Do-Yeal Ryu ₁ , Amena Khatun ₁ , Polash Chandra Karmakar ₁ , Buom-Yong Ryu ₁ , Myung-Geol Pang ₁

Affiliation

Studies regarding bisphenol A (BPA) exposure and male (in)fertility have conventionally focused on modifications in ejaculated spermatozoa function from exposed individuals. However, mammalian spermatozoa are incapable of fertilization prior to achieving capacitation, the penultimate step in maturation. Therefore, it is necessary to investigate BPA-induced changes in capacitated spermatozoa and assess the consequences on subsequent fertilization. Here, we demonstrate the effect of gestational BPA exposure (50 μg/kg bw/day, 5 mg/kg bw/day, and 50 mg/kg bw/day) on the functions, biochemical properties, and proteomic profiles of F1 capacitated spermatozoa from adult mice. The data showed that high concentrations of BPA inhibited motility, motion kinematics, and capacitation of spermatozoa, perhaps because of increased lipid peroxidation and protein tyrosine nitration, and decreased intracellular ATP levels and protein kinase-A activity in spermatozoa. We also found that BPA compromised the rates of fertilization and early embryonic development. Differentially expressed proteins identified between BPA-exposed and control groups play a critical role in energy metabolism, stress responses, and fertility. Protein function abnormalities were responsible for the development of several diseases according to bioinformatics analysis. On the basis of these results, gestational exposure to BPA may alter capacitated spermatozoa function and the proteomic profile, ultimately affecting their fertility potential.

中文翻译：

F1能力的成年小鼠受精暴露于双酚A后精子功能和蛋白质组学变化。

关于双酚A（BPA）暴露和男性（不育）生育能力的研究通常集中在暴露个体的射精精子功能改变上。但是，哺乳动物的精子不能在获能之前就受精，而获能是成熟的倒数第二个步骤。因此，有必要研究BPA诱导的精子能力丧失的变化，并评估其对随后受精的影响。在这里，我们证明了妊娠双酚A暴露（50μg/ kg bw /天，5 mg / kg bw /天和50 mg / kg bw /天）对F1致精子功能，生化特性和蛋白质组学特性的影响来自成年小鼠。数据表明，高浓度的BPA会抑制精子的运动，运动学和获能能力，可能是由于脂质过氧化作用增强和蛋白质酪氨酸硝化作用增加，以及精子中细胞内ATP水平和蛋白激酶A活性降低所致。我们还发现BPA损害了受精率和早期胚胎发育。在BPA暴露组和对照组之间鉴定出的差异表达蛋白在能量代谢，应激反应和生育力中起关键作用。根据生物信息学分析，蛋白质功能异常是导致多种疾病发展的原因。根据这些结果，妊娠期接触BPA可能会改变获能的精子功能和蛋白质组学特征，最终影响其生育能力。我们还发现BPA损害了受精率和早期胚胎发育。在BPA暴露组和对照组之间鉴定出的差异表达蛋白在能量代谢，应激反应和生育力中起关键作用。根据生物信息学分析，蛋白质功能异常是导致多种疾病发展的原因。根据这些结果，妊娠期接触BPA可能会改变获能的精子功能和蛋白质组学特征，最终影响其生育能力。我们还发现BPA损害了受精率和早期胚胎发育。在BPA暴露组和对照组之间鉴定出的差异表达蛋白在能量代谢，应激反应和生育力中起关键作用。根据生物信息学分析，蛋白质功能异常是导致多种疾病发展的原因。根据这些结果，妊娠期接触BPA可能会改变获能的精子功能和蛋白质组学特征，最终影响其生育能力。根据生物信息学分析，蛋白质功能异常是导致多种疾病发展的原因。根据这些结果，妊娠期接触BPA可能会改变获能的精子功能和蛋白质组学特征，最终影响其生育能力。根据生物信息学分析，蛋白质功能异常是导致多种疾病发展的原因。根据这些结果，妊娠期接触BPA可能会改变获能的精子功能和蛋白质组学特征，最终影响其生育能力。

更新日期：2017-12-22

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11