Hypochlorous acid (HOCl), a well-known universal disinfectant in clinical practice, plays important roles in immune systems of animal and human bodies. For understanding the roles of HOCl in living systems, a number of approaches, including chemiluminescence, colorimetric, electrochemical and chromatographic methods have been explored. For the detection of HOCl in live organisms, cutting-edge techniques, such as fluorescence/phosphorescence molecular probes, responsive nanoprobes, Raman and activatable photoacoustic sensors, have also been developed recently. In this review, the recent advances in the development of bioanalytical methods for detection of HOCl in environmental and biological specimens were summarized. More specifically, traditional techniques for assay of HOCl in bulk solution were initially discussed, and then fluorescence molecular probes, phosphorescence probes, responsive nanoprobes and other methods for HOCl detection were reviewed, which gives an overview of the developments and applications in bioanalytical methods for HOCl detection.

次氯酸（HOCl）是临床上众所周知的通用消毒剂，在动物和人体的免疫系统中起着重要的作用。为了理解HOC1在生命系统中的作用，已经探索了许多方法，包括化学发光，比色，电化学和色谱方法。为了检测活生物体中的HOCl，最近还开发了前沿技术，例如荧光/磷光分子探针，响应性纳米探针，拉曼光谱和可激活的光声传感器。在这篇综述中，总结了用于检测环境和生物样本中HOCl的生物分析方法的最新进展。更具体地说，最初讨论了用于测定本体溶液中HOCl的传统技术，