Phenolic compounds have been studied elaborately for their efficacy to improve health and to protect against a wide variety of diseases. Herein this study, different analysis methods were implemented to evaluate the antioxidant properties of catechin and cyanidin using their standard substances and as they found in the grape seeds extracts. Total phenol contents were 107.39 ± 8.94 mg GAE/g dw of grape seeds for grape seed extract (GSE) and 218.32 ± 10.66 mg GAE/g dw of grape seeds for acid-hydrolyzed grape seed extract (AcGSE). The extracts were analyzed by HPLC-DAD system and the results showed the presence of catechin, gallic acid, chlorogenic acid and ellagic acid in the processed methanolic extract and cyanidin, gallic acid and ellagic acid in the processed acidified methanolic extract. The protective abilities of catechin and cyanidin were tested against the oxidation of DNA. The results showed that cyanidin has better protection of DNA against oxidation than catechin. GSE and AcGSE were revealed to inhibit the oxidatively induced DNA damage. GSE decreased about 57% of damage caused by the Fenton control sample. This study could show new aspects of the antioxidant profiles of cyanidin and catechin.

已对酚类化合物改善健康和预防多种疾病的功效进行了详尽的研究。在本研究中，采用了不同的分析方法来评估儿茶素和花青素的抗氧化性能，这些方法使用的是它们的标准物质以及在葡萄籽提取物中所发现的。葡萄籽提取物（GSE）的总酚含量为107.39±8.94 mg GAE / g dw葡萄，酸水解葡萄籽提取物（AcGSE）的总苯酚含量为218.32±10.66 mg GAE / g dw葡萄籽。通过HPLC-DAD系统对提取物进行分析，结果显示在加工的甲醇提取物中存在儿茶素，没食子酸，绿原酸和鞣花酸，在加工的酸化的甲醇提取物中存在花青素，没食子酸和鞣花酸。测试了儿茶素和花青素对DNA氧化的保护能力。结果表明，与儿茶素相比，花青素对DNA的抗氧化保护作用更好。GSE和AcGSE被发现抑制氧化诱导的DNA损伤。GSE减少了Fenton对照样品造成的损坏的约57％。这项研究可以显示花青素和儿茶素的抗氧化特性的新方面。