Oil-in-salt hollow fiber liquid phase microextraction coupled with high performance liquid chromatography ultraviolet detection (HPLC-UV) was developed for determination of the blood concentrations of the main active compounds, hesperidin, honokiol, shikonin, magnolol, emodin and β,β’-dimethylacrylshikonin, after oral administration of Zi-Cao-Cheng-Qi decoction (ZCCQD) and their total plasma protein binding rates. In the procedure, a hollow fiber segment was immersed in organic solvent to fill the solvent in the fiber lumen and wall pore, and then the fiber was immersed into sodium chloride solution to cover a thin salt membrane on the fiber wall pore filling organic solvent. Various factors affecting the procedure, such as extraction solvent, sample phase pH, stirring rate, extraction time, NaCl concentration and fiber immersion time in the NaCl solution, were optimized. Under the optimum conditions, good linearities (r² ≥ 0.9905), low limits of detection (0.7–2.5 ng/mL) or quantitation (1.2–12 ng/mL), satisfactory precision (2.6%-12.8%) and accuracy (81.0%–114.2%) of this method, were observed. The results showed that, after oral administration of a 25 g/kg dose, (1) the blood concentrations (at 0.5 h) of hesperidin, honokiol, shikonin, magnolol, emodin and β,β’-dimethylacrylshikonin were 0.45, 0.40, 0.48, 0.74, 0.11 and 1.11 μg/mL, respectively; (2) the total plasma protein binding rates of the six active compounds were 42.0% (hesperidin), 71.8% (honokiol), 64.6% (shikonin), 77.7% (magnolol), 75.3% (emodin) and 75.7% (β,β’-dimethylacrylshikonin), respectively. The proposed procedure coupled with HPLC shows obvious advantages, such as low solvent consumption, simple operation, high sensitivity and strong purifying and can be used for the determination of both the blood concentrations and total plasma protein binding rates of active compounds in traditional Chinese medicine.

开发了盐包油中空纤维液相微萃取结合高效液相色谱紫外检测（HPLC-UV）来测定主要活性化合物橙皮苷，厚朴酚，紫草苷，厚朴酚，大黄素和β，β的血药浓度口服口服子草正气汤（ZCCQD）及其总血浆蛋白结合率后，'-二甲基丙烯酰胺紫草素。在该方法中，将中空纤维段浸入有机溶剂中以将溶剂填充在纤维腔和壁孔中，然后将纤维浸入氯化钠溶液中以覆盖纤维壁孔填充有机溶剂上的薄盐膜。影响程序的各种因素，例如萃取溶剂，样品相pH，搅拌速度，萃取时间，优化了NaCl浓度和纤维在NaCl溶液中的浸泡时间。在最佳条件下，良好的线性度（r^2个 ≥0.9905），此方法的检测下限（0.7–2.5 ng / mL）或定量（1.2–12 ng / mL），满意的精密度（2.6％-12.8％）和准确度（81.0％–114.2％）是观察到的。结果显示，以25 g / kg的剂量口服后，（1）橙皮苷，厚朴酚，紫草素，厚朴酚，大黄素，大黄素和β，β'-二甲基丙烯酸紫草素的血药浓度（0.5小时）分别为0.45、0.40、0.48分别为0.74、0.11和1.11μg/ mL；（2）六种活性化合物的总血浆蛋白结合率分别为42.0％（橙皮苷），71.8％（厚朴酚），64.6％（紫草素），77.7％（厚朴酚），75.3％（大黄素）和75.7％（β， β'-二甲基丙烯酸紫草素）。所建议的方法与HPLC结合显示出明显的优势，例如溶剂消耗低，操作简单，