In this study, the anaerobic digestion of nitrogen-rich chicken manure from egg-laying hens was investigated via long-term continuous experiments with and without the addition of different trace elements. With trace element supplementation, a CH₄ yield of 0.26 ± 0.03 m³ kg⁻¹ of volatile solids (VS) added was achieved at an organic loading rate (OLR) of 3.62 kg m⁻³ day⁻¹ based on VS and total ammonia nitrogen (TAN) content greater than 7200 g m⁻³. Selenium (Se) was identified as the critical trace element for the stable anaerobic digestion of chicken manure. The dominant methanogen in the reactors was the hydrogenotrophic methanogen Methanoculleus bourgensis. Therefore, we concluded that at elevated TAN concentrations, the CH₄ production stimulated by Se supplementation likely occurred through syntrophic acetate oxidation. Without trace element supplementation, severe acetic and propionic acid accumulation occurred, causing the CH₄ yield to decrease below 0.12 m³ kg⁻¹ of VS added.

在这项研究中，通过长期连续实验，在有和没有添加不同微量元素的情况下，对产蛋母鸡中富氮鸡粪的厌氧消化进行了研究。通过添加微量元素，在基于有机硅和总氨的有机负载率（OLR）为3.62 kg m ^-3天^-1的情况下，添加的CH ₄产量为0.26±0.03 m ³  kg ^-1挥发性固体（VS）氮（TAN）含量大于7200 gm ^-3。硒（Se）被确定为鸡粪稳定厌氧消化的关键微量元素。反应器中主要的产甲烷菌是氢营养型产甲烷菌甲烷菌。因此，我们得出的结论是，在较高的TAN浓度下，由硒补充引起的CH ₄产生可能是通过乙酸同养型氧化产生的。在不添加微量元素的情况下，会发生大量的乙酸和丙酸积聚，导致CH ₄产量降至添加的VS的0.12 m ³  kg ^-1以下。