Do protocells preferentially retain macromolecular solutes upon division/fragmentation? A study based on the extrusion of POPC giant vesicles,Integrative Biology

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Do protocells preferentially retain macromolecular solutes upon division/fragmentation? A study based on the extrusion of POPC giant vesicles
Integrative Biology ( IF 1.5 ) Pub Date : 2017-12-12 , DOI: 10.1039/c7ib00138j
Alessio Fanti _{1,

2,

3} , Leandro Gammuto _{1,

2,

3} , Fabio Mavelli _{3,

4,

5} , Pasquale Stano _{3,

6,

7} , Roberto Marangoni _{1,

2,

3,

8,

9}

Affiliation

A key process of protocell behaviour is their recursive growth and division. In order to be sustainable, the latter must be characterized by an even and homogeneous partition of the solute molecules initially present in the parent protocell among the daughter ones. Here we have investigated, by means of an artificial division model (extrusion of giant lipid vesicles) and confocal microscopy, the fate of solutes when a large vesicle fragments into many smaller vesicles. Solutes of low- and high-molecular weight such as pyranine, calcein, albumin-FITC, dextran—FITC and carbonic anhydrase have been employed. Although the vesicle extrusion brings about a release of their inner content in the environment, the results shown in this initial report indicate that macromolecules can be partially retained when compared with low-molecular weight ones. Results are discussed from the viewpoint of the life cycle of primitive cells. In particular, the findings suggest that a similar mechanism operating during the critical step of vesicle growth—division could have contributed to primitive evolution.

中文翻译：

原生细胞在分裂/片段化后是否优先保留大分子溶质？基于POPC大囊泡挤压的研究

原始细胞行为的关键过程是它们的递归生长和分裂。为了具有可持续性，后者的特征必须是最初存在于母细胞中的溶质分子在子细胞中的均匀均匀分布。在这里，我们已经通过人工分裂模型（巨大脂质囊泡的挤出）和共聚焦显微镜研究了当大囊泡破碎成许多较小囊泡时溶质的命运。已经使用了低分子量和高分子量的溶质，例如吡喃酸，钙黄绿素，白蛋白-FITC，右旋糖酐-FITC和碳酸酐酶。尽管囊泡的挤出会释放环境中的内含物，但该初始报告中显示的结果表明，与低分子量的大分子相比，大分子可以部分保留。从原始细胞生命周期的角度讨论了结果。特别是，研究结果表明，在囊泡生长的关键步骤（分裂）中起作用的类似机制可能有助于原始进化。

更新日期：2017-12-12

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