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Intracellular investigation on the differential effects of 4 polyphenols on MCF-7 breast cancer cells by Raman imaging
Analyst ( IF 3.6 ) Pub Date : 2017-12-01 00:00:00 , DOI: 10.1039/c7an01460k A. Mignolet 1, 2, 3, 4, 5 , B. R. Wood 6, 7, 8, 9 , E. Goormaghtigh 1, 2, 3, 4, 5
Analyst ( IF 3.6 ) Pub Date : 2017-12-01 00:00:00 , DOI: 10.1039/c7an01460k A. Mignolet 1, 2, 3, 4, 5 , B. R. Wood 6, 7, 8, 9 , E. Goormaghtigh 1, 2, 3, 4, 5
Affiliation
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The past decades have seen significant interest in the study of polyphenolic compounds as potential therapeutic agents in medicine because they display a vast array of cellular effects beneficial to treat or manage a plethora of chronic diseases including inflammatory diseases, cardiovascular abnormalities and several types of cancer. These compounds act at different stages of carcinogenesis but deciphering their mode of action is a complex task. Live MCF-7 breast cancer cells were investigated using Raman imaging to evaluate the perturbations induced after incubating cells with four different polyphenols: EGCG, gallic acid, resveratrol and tannic acid. First, clear spectral changes could be observed between the spectra of the cytoplasm and the nucleus of live MCF-7 cancer cells demonstrating a difference in their respective global chemical composition. The treatments induced significant modifications in the cells but no clear common pattern of modifications from the 4 drugs could be observed in the cell spectra in the 1800–600 cm−1 region. The high spatial resolution of Raman confocal microscopy enabled both the nucleus and cytoplasm to be independently targeted to study the impact of the polyphenols on the cell line. Positive spectral variations at 2851 cm−1 and 2920 cm−1 as well as in the 1460–1420 cm−1 and 1660–1650 cm−1 spectral regions inside cell cytoplasm reflected an increase of the lipid content after exposure to polyphenols. Lipid accumulation appears to be an early biomarker of drug-induced cell stress and subsequent apoptosis. Interestingly an increase of cytochrome c into the cytosol was also induced by EGCG. These multiple events are possibly associated with cell apoptosis. In conclusion, Raman micro-spectroscopy provides a complementary spectroscopic method to realize biological investigations on live cancer cells and to evaluate the effects of polyphenols at the subcellular level.
中文翻译：
拉曼成像细胞内研究4种多酚对MCF-7乳腺癌细胞的差异作用
在过去的几十年中，人们对多酚化合物作为药物的潜在治疗剂的研究产生了浓厚的兴趣，因为它们显示出广泛的细胞效应，有利于治疗或控制多种慢性疾病，包括炎性疾病，心血管异常和几种类型的癌症。这些化合物在癌变的不同阶段起作用，但是解密它们的作用方式是一项复杂的任务。使用拉曼成像研究了活的MCF-7乳腺癌细胞，以评估将细胞与四种不同的多酚（EGCG，没食子酸，白藜芦醇和单宁酸）孵育后引起的扰动。第一的，在活的MCF-7癌细胞的细胞质和细胞核的光谱之间可以观察到明显的光谱变化，这表明它们各自的整体化学组成有所不同。这些处理诱导了细胞的显着修饰，但是在1800-600 cm的细胞光谱中未观察到四种药物的明显修饰模式-1区。拉曼共聚焦显微镜的高空间分辨率使细胞核和细胞质都可以独立定位，以研究多酚对细胞系的影响。在2851 cm -1和2920 cm -1以及1460-1420 cm -1和1660-1650 cm -1处的正光谱变化细胞质内的光谱区域反映了暴露于多酚后脂质含量的增加。脂质蓄积似乎是药物诱导的细胞应激和随后的细胞凋亡的早期生物标志物。有趣的是，EGCG也诱导细胞色素c进入细胞质。这些多重事件可能与细胞凋亡有关。总之，拉曼光谱法提供了一种补充光谱法，可用于对活癌细胞进行生物学研究并评估多酚在亚细胞水平上的作用。
更新日期：2017-12-01
中文翻译：
拉曼成像细胞内研究4种多酚对MCF-7乳腺癌细胞的差异作用
在过去的几十年中，人们对多酚化合物作为药物的潜在治疗剂的研究产生了浓厚的兴趣，因为它们显示出广泛的细胞效应，有利于治疗或控制多种慢性疾病，包括炎性疾病，心血管异常和几种类型的癌症。这些化合物在癌变的不同阶段起作用，但是解密它们的作用方式是一项复杂的任务。使用拉曼成像研究了活的MCF-7乳腺癌细胞，以评估将细胞与四种不同的多酚（EGCG，没食子酸，白藜芦醇和单宁酸）孵育后引起的扰动。第一的，在活的MCF-7癌细胞的细胞质和细胞核的光谱之间可以观察到明显的光谱变化，这表明它们各自的整体化学组成有所不同。这些处理诱导了细胞的显着修饰，但是在1800-600 cm的细胞光谱中未观察到四种药物的明显修饰模式-1区。拉曼共聚焦显微镜的高空间分辨率使细胞核和细胞质都可以独立定位，以研究多酚对细胞系的影响。在2851 cm -1和2920 cm -1以及1460-1420 cm -1和1660-1650 cm -1处的正光谱变化细胞质内的光谱区域反映了暴露于多酚后脂质含量的增加。脂质蓄积似乎是药物诱导的细胞应激和随后的细胞凋亡的早期生物标志物。有趣的是，EGCG也诱导细胞色素c进入细胞质。这些多重事件可能与细胞凋亡有关。总之，拉曼光谱法提供了一种补充光谱法，可用于对活癌细胞进行生物学研究并评估多酚在亚细胞水平上的作用。
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