Microprinted Stem Cell Niches Reveal Compounding Effect of Colony Size on Stromal Cells‐Mediated Neural Differentiation,Advanced Healthcare Materials

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Microprinted Stem Cell Niches Reveal Compounding Effect of Colony Size on Stromal Cells‐Mediated Neural Differentiation
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2017-11-30 , DOI: 10.1002/adhm.201700832
Ramila Joshi ₁ , Pradip Shahi Thakuri ₁ , James C Buchanan ₁ , Jun Li ₂ , Hossein Tavana ₃

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Microenvironmental factors have a major impact on differentiation of embryonic stem cells (ESCs). Here, a novel phenomenon that size of ESC colonies has a significant regulatory role on stromal cells induced differentiation of ESCs to neural cells is reported. Using a robotic cell microprinting technology, defined densities of ESCs are confined within aqueous nanodrops over a layer of supporting stromal cells immersed in a second, immiscible aqueous phase to generate ESC colonies of defined sizes. Temporal protein and gene expression studies demonstrate that larger ESC colonies generate disproportionally more neural cells and longer neurite processes. Unlike previous studies that attribute neural differentiation of ESCs solely to interactions with stromal cells, it is found that increased intercellular signaling of ESCs significantly enhances neural differentiation. This study offers an approach to generate neural cells with improved efficiency for potential use in translational research.

中文翻译：

微印刷干细胞生态位揭示集落大小对基质细胞介导的神经分化的复合效应

微环境因素对胚胎干细胞（ESC）的分化有重大影响。在此，报道了一种新现象，即 ESC 集落的大小对基质细胞诱导 ESC 向神经细胞的分化具有显着的调节作用。使用机器人细胞微印刷技术，将特定密度的 ESC 限制在水性纳米滴内，覆盖在浸入第二个不混溶水相中的支撑基质细胞层上，以生成特定大小的 ESC 集落。时间蛋白和基因表达研究表明，较大的 ESC 集落会产生不成比例的更多神经细胞和更长的神经突。之前的研究将ESC的神经分化仅仅归因于与基质细胞的相互作用，与此不同的是，研究发现ESC细胞间信号传导的增加可显着增强神经分化。这项研究提供了一种以更高的效率生成神经细胞的方法，可用于转化研究。

更新日期：2017-11-30

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