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Differential Expression of MicroRNAs in Embryos and Larvae of Mahi-Mahi (Coryphaena hippurus) Exposed to Deepwater Horizon Oil
Environmental Science & Technology Letters ( IF 8.9 ) Pub Date : 2017-11-29 00:00:00 , DOI: 10.1021/acs.estlett.7b00484 Graciel Diamante 1 , Elvis Genbo Xu 1 , Shuai Chen 2 , Edward Mager 3, 4 , Martin Grosell 4 , Daniel Schlenk 1
Environmental Science & Technology Letters ( IF 8.9 ) Pub Date : 2017-11-29 00:00:00 , DOI: 10.1021/acs.estlett.7b00484 Graciel Diamante 1 , Elvis Genbo Xu 1 , Shuai Chen 2 , Edward Mager 3, 4 , Martin Grosell 4 , Daniel Schlenk 1
Affiliation
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Deepwater Horizon (DWH) oil causes developmental cardiotoxicity in fish species, but the molecular mechanisms are still not well understood. MicroRNAs (miRNAs) play key roles in a number of biological processes, including heart development. Therefore, the expression of miRNAs in mahi-mahi (Coryphaena hippurus) exposed to weathered slick oil and nonweathered source oil was analyzed. miRNAs were sequenced and annotated using the Fugu rubripes genome (termed the Fugu-guided approach) or aligned with known mature animal miRNAs using the Basic Local Alignment Search Tool (BLAST) method (termed the phylogenetically guided approach). Exposure of embryos to slick oil resulted in more differentially expressed miRNAs than exposure to source oil at all developmental stages. Gene ontology analysis on the target mRNAs was consistent with pathway analysis of miRNAs, predicting disruption of cardiovascular system development after oil exposure, and specific miRNA–mRNA interactions identified by ingenuity pathway analysis. Slick oil caused an overexpression of miR-133, which correlated with the decrease in the level of expression of genes related to the cardiovascular system such as potassium voltage-gated channel subfamily H member 2 (kchn2). This work is the first study linking miRNAs and mRNAs in crude oil-exposed fish, providing a new understanding of the molecular mechanism(s) of oil toxicity.
中文翻译：
暴露于深水地平线油中的Mahi-Mahi（Coryphaena hippurus）的胚和幼虫中microRNA的差异表达
深水地平线（DWH）油对鱼类具有发展性的心脏毒性作用，但其分子机理仍未得到很好的了解。MicroRNA（miRNA）在包括心脏发育在内的许多生物学过程中起着关键作用。因此，分析了miRNA在暴露于风化的浮油和非风化的源油中的Mahi -mahi（Coryphaena hippurus）中的表达。使用Fugu rubripes基因组（称为Fugu）对miRNA进行测序和注释引导方法）或使用基本局部比对搜索工具（BLAST）方法与已知的成熟动物miRNA进行比对（称为系统发育指导方法）。在所有发育阶段，暴露于浮油的胚胎所产生的miRNA差异要比暴露于源油的差异更大。对靶mRNA的基因本体分析与miRNA的途径分析相符，预测了机油暴露后心血管系统发育的破坏，以及通过机巧途径分析确定的特定的miRNA-mRNA相互作用。浮油导致miR-133的过表达，这与与心血管系统相关的基因（如钾电压门控通道亚家族H成员2（kchn2）。这项工作是首次将暴露于原油的鱼类中的miRNA和mRNA连接起来的研究，为对油中毒的分子机理提供了新的认识。
更新日期：2017-11-30
中文翻译：
暴露于深水地平线油中的Mahi-Mahi（Coryphaena hippurus）的胚和幼虫中microRNA的差异表达
深水地平线（DWH）油对鱼类具有发展性的心脏毒性作用，但其分子机理仍未得到很好的了解。MicroRNA（miRNA）在包括心脏发育在内的许多生物学过程中起着关键作用。因此，分析了miRNA在暴露于风化的浮油和非风化的源油中的Mahi -mahi（Coryphaena hippurus）中的表达。使用Fugu rubripes基因组（称为Fugu）对miRNA进行测序和注释引导方法）或使用基本局部比对搜索工具（BLAST）方法与已知的成熟动物miRNA进行比对（称为系统发育指导方法）。在所有发育阶段，暴露于浮油的胚胎所产生的miRNA差异要比暴露于源油的差异更大。对靶mRNA的基因本体分析与miRNA的途径分析相符，预测了机油暴露后心血管系统发育的破坏，以及通过机巧途径分析确定的特定的miRNA-mRNA相互作用。浮油导致miR-133的过表达，这与与心血管系统相关的基因（如钾电压门控通道亚家族H成员2（kchn2）。这项工作是首次将暴露于原油的鱼类中的miRNA和mRNA连接起来的研究，为对油中毒的分子机理提供了新的认识。
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