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Part by Part: Synthetic Biology Parts Used in Solventogenic Clostridia
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2017-12-11 00:00:00 , DOI: 10.1021/acssynbio.7b00327 Ivan S. Gyulev 1 , Benjamin J. Willson 1 , Rosanna C. Hennessy 2 , Preben Krabben 3 , Elizabeth R. Jenkinson 3 , Gavin H. Thomas 1
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2017-12-11 00:00:00 , DOI: 10.1021/acssynbio.7b00327 Ivan S. Gyulev 1 , Benjamin J. Willson 1 , Rosanna C. Hennessy 2 , Preben Krabben 3 , Elizabeth R. Jenkinson 3 , Gavin H. Thomas 1
Affiliation
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The solventogenic Clostridia are of interest to the chemical industry because of their natural ability to produce chemicals such as butanol, acetone and ethanol from diverse feedstocks. Their use as whole cell factories presents multiple metabolic engineering targets that could lead to improved sustainability and profitability of Clostridium industrial processes. However, engineering efforts have been held back by the scarcity of genetic and synthetic biology tools. Over the past decade, genetic tools to enable transformation and chromosomal modifications have been developed, but the lack of a broad palette of synthetic biology parts remains one of the last obstacles to the rapid engineered improvement of these species for bioproduction. We have systematically reviewed existing parts that have been used in the modification of solventogenic Clostridia, revealing a narrow range of empirically chosen and nonengineered parts that are in current use. The analysis uncovers elements, such as promoters, transcriptional terminators and ribosome binding sites where increased fundamental knowledge is needed for their reliable use in different applications. Together, the review provides the most comprehensive list of parts used and also presents areas where an improved toolbox is needed for full exploitation of these industrially important bacteria.
中文翻译：
分部：溶剂型梭状芽胞杆菌中使用的合成生物学部件
产溶剂的梭状芽胞杆菌由于其天然的能力从多种原料生产丁醇，丙酮和乙醇等化学物质而引起化学工业的兴趣。它们作为全细胞工厂的用途提出了多种代谢工程学目标，可能会提高梭菌的可持续性和盈利能力工业过程。但是，由于缺乏遗传和合成生物学工具，工程工作受到了阻碍。在过去的十年中，已经开发了实现转化和染色体修饰的遗传工具，但是缺乏广泛的合成生物学部分仍然是对这些物种进行快速工程改良以用于生物生产的最后障碍之一。我们已系统地审查了已用于溶剂型梭菌改造的现有零件，揭示了目前使用的经验选择和非工程零件的范围很窄。该分析发现了诸如启动子，转录终止子和核糖体结合位点等元件，这些元件需要更多的基础知识才能在不同应用中可靠使用。一起，
更新日期：2017-12-11
中文翻译：
分部：溶剂型梭状芽胞杆菌中使用的合成生物学部件
产溶剂的梭状芽胞杆菌由于其天然的能力从多种原料生产丁醇，丙酮和乙醇等化学物质而引起化学工业的兴趣。它们作为全细胞工厂的用途提出了多种代谢工程学目标，可能会提高梭菌的可持续性和盈利能力工业过程。但是，由于缺乏遗传和合成生物学工具，工程工作受到了阻碍。在过去的十年中，已经开发了实现转化和染色体修饰的遗传工具，但是缺乏广泛的合成生物学部分仍然是对这些物种进行快速工程改良以用于生物生产的最后障碍之一。我们已系统地审查了已用于溶剂型梭菌改造的现有零件，揭示了目前使用的经验选择和非工程零件的范围很窄。该分析发现了诸如启动子，转录终止子和核糖体结合位点等元件，这些元件需要更多的基础知识才能在不同应用中可靠使用。一起，
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