Collagen, processed into several morphologies and originating from various sources, has long since been used as a biocompatible material that can assist wound healing and tissue regeneration. With the advent of biotechnology and solid-phase peptide synthesis, new possibilities arise to create rationally designed biomaterials based on collagen sequences incorporating new functionalities while maintaining the beneficial properties of natural collagen. In this study a new class of synthetic collagen materials is presented, defined by its simplistic core structure and its therefore predictable behavior. These so-called eCols (engineered collagens) consist of a varying number of Gly-Pro-Pro repeats, a redox-switchable aminoterminal nucleation site and an optional carboxyterminal cell adhesion motif. We show which of these proteins are able to self-assemble into triple helices and cross-linked gelatinous networks and provide insights into their cytocompatibility in vitro.

中文翻译：

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工程化的胶原蛋白：可氧化装配的可转换框架，用于可调节组装和生物相容性表面的制造

长期以来，胶原蛋白被加工成多种形态并起源于多种来源，一直被用作生物相容性材料，可以帮助伤口愈合和组织再生。随着生物技术和固相肽合成的出现，出现了新的可能性，以基于胶原序列的合理设计的生物材料结合了新的功能，同时又保持了天然胶原的有益特性。在这项研究中，提出了一类新的合成胶原材料，其定义是其核心结构简单，因此可预测行为。这些所谓的eCols（工程胶原蛋白）由不同数量的Gly-Pro-Pro重复序列，氧化还原可转换的氨基末端成核位点和可选的羧基末端细胞粘附基序组成。