Comparative Proteomics of Enterotoxigenic Escherichia coli Reveals Differences in Surface Protein Production and Similarities in Metabolism,Journal of Proteome Research

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Comparative Proteomics of Enterotoxigenic Escherichia coli Reveals Differences in Surface Protein Production and Similarities in Metabolism
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2017-12-12 00:00:00 , DOI: 10.1021/acs.jproteome.7b00593
Veronika Kuchařová Pettersen ₁ , Hans Steinsland ₁ , Harald G. Wiker ₁

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Enterotoxigenic Escherichia coli (ETEC) infections are an important cause of diarrhea among young children living in low- and middle-income countries and visiting travelers. The development of effective vaccines is complicated by substantial genomic diversity that exists among ETEC isolates. To investigate how ETEC genomic variation is reflected at expressed proteome level, we applied label-free quantitative proteomics to seven human ETEC strains representing five epidemiologically important lineages. We further determined the proteome profile of the nonpathogenic E. coli B strain BL21(DE3) to discriminate features specific for ETEC. The analysis yielded a data set of 2893 proteins, of which 1729 were present in all strains. Each ETEC strain produced on average 27 plasmid- or chromosomally-encoded proteins with known or putative connections to virulence, and a number of strain-specific proteins associated with the biosynthesis of surface antigens. Statistical comparison of protein levels between the ETEC strains and BL21(DE3) revealed several proteins with considerably increased levels only in BL21(DE3) including enzymes of arginine biosynthesis and metabolism of melibiose, galactitol, and gluconate. ETEC strains displayed consistently increased levels of proteins that were functional in iron acquisition, maltose metabolism, and acid resistance. The latter results suggest that specific metabolic functions might be shared among ETEC isolates.

中文翻译：

产肠毒素大肠杆菌的比较蛋白质组学揭示了表面蛋白产生和代谢相似性的差异

肠毒素性大肠杆菌（ETEC）感染是生活在中低收入国家和来访旅行者中的幼儿腹泻的重要原因。有效疫苗的开发由于ETEC分离株之间存在大量的基因组多样性而变得复杂。为了研究ETEC基因组变异如何在表达的蛋白质组水平上得到反映，我们将无标签定量蛋白质组学应用于代表五个流行病学重要谱系的七种人类ETEC菌株。我们进一步确定了非致病性大肠杆菌的蛋白质组谱B株BL21（DE3）区分ETEC特有的特征。该分析产生了2893个蛋白质的数据集，其中所有菌株中都存在1729个蛋白质。每个ETEC菌株平均产生27种质粒或染色体编码的蛋白，这些蛋白与毒力具有已知或假定的联系，还产生了许多与表面抗原生物合成相关的菌株特异性蛋白。ETEC菌株和BL21（DE3）之间蛋白质水平的统计比较表明，只有在BL21（DE3）中，几种蛋白质的含量才大大增加，包括精氨酸的生物合成酶和半乳糖，半乳糖和葡萄糖酸的代谢。ETEC菌株显示出不断增加的蛋白质水平，这些蛋白质在铁的摄取，麦芽糖的代谢和抗酸性方面发挥作用。

更新日期：2017-12-12

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