Stabilization of Multimeric Enzymes against Heat Inactivation by Chitosan-graft-poly(N-isopropylacrylamide) in Confined Spaces,ACS Biomaterials Science & Engineering

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Stabilization of Multimeric Enzymes against Heat Inactivation by Chitosan-graft-poly(N-isopropylacrylamide) in Confined Spaces
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2017-11-30 00:00:00 , DOI: 10.1021/acsbiomaterials.7b00764
Qian Tao ₁ , Ang Li , Zhenkun Zhang , Rujiang Ma , Linqi Shi ₁

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The inactivation of multimeric enzymes is a more complicated process compared with that of monomeric enzymes. Stabilization of multimeric enzymes is regarded as a challenge with practical values in enzyme technology. Temperature-sensitive copolymer chitosan-graft- poly(N-isopropylacrylamide) was synthesized and encapsulated with multimeric enzymes in the confined spaces constructed by the W/O microemulsion. In this way, the quaternary structures of multimeric enzymes are stabilized and the thermal stabilities of them are enhanced. The whole process was studied and discussed. This method, which works well for both glucose oxidase and catalase, can be developed as a general protection strategy for multimeric enzymes.

中文翻译：

壳聚糖-接枝-聚(N-异丙基丙烯酰胺)在密闭空间中对多聚酶热失活的稳定性

与单体酶相比，多聚体酶的失活是一个更为复杂的过程。多聚体酶的稳定化被认为是酶技术中具有实用价值的挑战。合成了壳聚糖-接枝-聚( N-异丙基丙烯酰胺)的温度敏感共聚物，并在W/O微乳液构建的密闭空间中用多聚酶包封。这样，多聚酶的四级结构得到稳定，它们的热稳定性得到增强。对整个过程进行了研究和讨论。这种对葡萄糖氧化酶和过氧化氢酶都有效的方法可以开发为多聚酶的通用保护策略。

更新日期：2017-11-30

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