Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
On Chip Protein Pre-Concentration for Enhancing the Sensitivity of Porous Silicon Biosensors
ACS Sensors ( IF 8.2 ) Pub Date : 2017-11-27 00:00:00 , DOI: 10.1021/acssensors.7b00692 Sofia Arshavsky-Graham 1 , Naama Massad-Ivanir , Federico Paratore 2 , Thomas Scheper 1 , Moran Bercovici , Ester Segal
ACS Sensors ( IF 8.2 ) Pub Date : 2017-11-27 00:00:00 , DOI: 10.1021/acssensors.7b00692 Sofia Arshavsky-Graham 1 , Naama Massad-Ivanir , Federico Paratore 2 , Thomas Scheper 1 , Moran Bercovici , Ester Segal
Affiliation
|
Porous silicon (PSi) nanomaterials have been widely studied as label-free optical biosensors for protein detection. However, these biosensors’ performance, specifically in terms of their sensitivity (which is typically in the micromolar range), is insufficient for many applications. Herein, we present a proof-of-concept application of the electrokinetic isotachophoresis (ITP) technique for real-time preconcentration of a target protein on a PSi biosensor. With ITP, a highly concentrated target zone is delivered to the sensing area, where the protein target is captured by immobilized aptamers. The detection of the binding events is conducted in a label-free manner by reflective interferometric Fourier transformation spectroscopy (RIFTS). Up to 1000-fold enhancement in local concentration of the protein target and the biosensor’s sensitivity are achieved, with a measured limit of detection of 7.5 nM. Furthermore, the assay is successfully performed in complex media, such as bacteria lysate samples, while the selectivity of the biosensor is retained. The presented assay could be further utilized for other protein targets, and to promote the development of clinically useful PSi biosensors.
中文翻译：
芯片上蛋白质预浓缩可增强多孔硅生物传感器的灵敏度
多孔硅（PSi）纳米材料已被广泛用作蛋白质检测的无标记光学生物传感器。然而，这些生物传感器的性能，特别是就其灵敏度而言（通常在微摩尔范围内）对于许多应用来说是不够的。在本文中，我们介绍了电动等速电泳（ITP）技术在PSi生物传感器上实时预浓缩目标蛋白的概念验证应用。借助ITP，高浓度的靶标区域将被传递到传感区域，在该区域中蛋白质靶标被固定的适体捕获。结合事件的检测通过反射干涉傅里叶变换光谱法（RIFTS）以无标记的方式进行。蛋白质靶标的局部浓度和生物传感器的灵敏度最高可提高1000倍，检测极限为7.5 nM。此外，在保留生物传感器的选择性的同时，可以在复杂的介质（例如细菌裂解液样品）中成功进行测定。提出的测定法可进一步用于其他蛋白质靶标，并促进临床上有用的PSi生物传感器的开发。
更新日期：2017-11-28
中文翻译：
芯片上蛋白质预浓缩可增强多孔硅生物传感器的灵敏度
多孔硅（PSi）纳米材料已被广泛用作蛋白质检测的无标记光学生物传感器。然而，这些生物传感器的性能，特别是就其灵敏度而言（通常在微摩尔范围内）对于许多应用来说是不够的。在本文中，我们介绍了电动等速电泳（ITP）技术在PSi生物传感器上实时预浓缩目标蛋白的概念验证应用。借助ITP，高浓度的靶标区域将被传递到传感区域，在该区域中蛋白质靶标被固定的适体捕获。结合事件的检测通过反射干涉傅里叶变换光谱法（RIFTS）以无标记的方式进行。蛋白质靶标的局部浓度和生物传感器的灵敏度最高可提高1000倍，检测极限为7.5 nM。此外，在保留生物传感器的选择性的同时，可以在复杂的介质（例如细菌裂解液样品）中成功进行测定。提出的测定法可进一步用于其他蛋白质靶标，并促进临床上有用的PSi生物传感器的开发。
京公网安备 11010802027423号