Nucleic acid templated reactions have attracted attention as an important technology to sense oligonucleotides and to translate nucleic acid-based instructions into diverse outputs. Great progress has been made in accelerating the reaction in order to improve signal amplification, reaching rates where substrate turnover rather than chemical reaction is rate limiting. Herein we explore the utility of architectures inspired by three-way junction that yield a cleavage of a strand thus accelerating substrate turnover. We demonstrate that such design can overcome product inhibition in templated reactions and operate close to the rate of hybridization.

中文翻译：

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在核酸模板反应中加快周转频率

核酸模板化反应作为一种重要的技术已引起人们的注意，该技术可检测寡核苷酸并将基于核酸的指令翻译成多种输出。在加速反应以改善信号放大方面已经取得了很大的进步，达到了速率，其中底物周转而不是化学反应是速率的限制。在本文中，我们探索了受三向连接启发的体系结构的实用性，这种连接产生了一条链的切割，从而加速了底物的转化。我们证明，这种设计可以克服模板反应中的产物抑制作用，并以接近杂交的速率运行。