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Effect of Site‐Specific Peptide‐Tag Labeling on the Biocatalytic Properties of Thermoalkalophilic Lipase from Geobacillus thermocatenulatus
ChemBioChem ( IF 2.6 ) Pub Date : 2018-01-04 , DOI: 10.1002/cbic.201700466
Oscar Romero 1 , Blanca de las Rivas 2 , David Lopez-Tejedor 1 , Jose M. Palomo 1
Affiliation  

New semisynthetic lipases: Site‐specific chemical incorporation of cysteine‐containing peptide tags on the lid site of cysteine‐genetically modified thermoalkalophilic lipase variants is developed. New modified peptide–lipase conjugates with improved activity, specificity, and selectivity are prepared.
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中文翻译:

特定位点肽标签标记对嗜热地热芽孢杆菌嗜热碱脂肪酶生物催化特性的影响

新的半合成脂肪酶:开发了在半胱氨酸基因修饰的热嗜碱脂肪酶变体的盖子位点上特定位置的半胱氨酸肽标记的化学结合方法。制备了具有改进的活性,特异性和选择性的新型修饰的肽-脂肪酶偶联物。
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更新日期:2018-01-04
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