Coexisting properties of thermostability and ultraviolet radiation resistance in the main S-layer complex of Deinococcus radiodurans†,Photochemical & Photobiological Sciences

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Coexisting properties of thermostability and ultraviolet radiation resistance in the main S-layer complex of Deinococcus radiodurans†
Photochemical & Photobiological Sciences ( IF 2.7 ) Pub Date : 2017-11-27 00:00:00 , DOI: 10.1039/c7pp00240h
Domenica Farci _{1,

2,

3,

4,

5} , Chavdar Slavov _{6,

7,

8,

9} , Dario Piano _{1,

2,

3,

4,

5}

Affiliation

Deinococcus radiodurans is well known for its unusual resistance to different environmental stresses. Recently, we have described a novel complex composed of the surface (S)-layer protein DR_2577 and the carotenoid deinoxanthin. We also showed a role of this complex in the UV resistance under desiccation. Both these properties, UV and desiccation resistance, suggest a selective pressure generated by Sun irradiation. In order to confirm this hypothesis we checked whether this S-layer Deinoxanthin Binding Complex (SDBC) has features of thermo-resistance, a property also expected in proteins evolved under solar irradiative pressure. We performed the spectroscopic characterization of the SDBC by means of thermal shift assay, circular dichroism and related in silico analysis. Our findings identify a stability typical of thermo-adapted proteins and provide a new insight into the origin of specific S-layer types. The results are discussed in terms of co-evolutionary mechanisms related to Sun-induced desiccation and heat.

中文翻译：

共存的热稳定性和耐紫外线特性的性质的主要S-层复合耐辐射奇球菌†

放射球菌（Deinococcus radiodurans）以其对不同环境压力的异常抵抗能力而闻名。最近，我们描述了一种由表面（S）层蛋白DR_2577和类胡萝卜素脱黄嘌呤组成的新型复合物。我们还显示了这种复合物在干燥下的抗紫外线性中的作用。这两个特性（抗紫外线和抗干燥性）都表明太阳辐射会产生选择性压力。为了证实这一假设，我们检查了这种S层脱氧黄嘌呤结合复合物（SDBC）是否具有耐热特性，该特性也是在太阳辐射压力下进化出的蛋白质中所期望的。我们通过热位移分析，圆二色性和相关的计算机模拟对SDBC进行了光谱表征分析。我们的发现确定了热适应蛋白的典型稳定性，并为特定S层类型的起源提供了新的见识。根据与太阳引起的干燥和热有关的共同进化机制对结果进行了讨论。

更新日期：2017-11-27

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