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Proteome Comparisons between Hemolymph of Two Honeybee Strains (Apis mellifera ligustica) Reveal Divergent Molecular Basis in Driving Hemolymph Function and High Royal Jelly Secretion
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2017-12-05 00:00:00 , DOI: 10.1021/acs.jproteome.7b00621 Zewdu Ararso 1 , Chuan Ma 1 , Yuping Qi 1 , Mao Feng 1 , Bin Han 1 , Han Hu 1 , Lifeng Meng 1 , Jianke Li 1
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2017-12-05 00:00:00 , DOI: 10.1021/acs.jproteome.7b00621 Zewdu Ararso 1 , Chuan Ma 1 , Yuping Qi 1 , Mao Feng 1 , Bin Han 1 , Han Hu 1 , Lifeng Meng 1 , Jianke Li 1
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Hemolymph is vital for the immunity of honeybees and offers a way to investigate their physiological status. To gain novel insight into the functionality and molecular details of the hemolymph in driving increased Royal Jelly (RJ) production, we characterized and compared hemolymph proteomes across the larval and adult ages of Italian bees (ITbs) and Royal Jelly bees (RJbs), a stock selected from ITbs for increasing RJ output. Unprecedented in-depth proteome was attained with the identification of 3394 hemolymph proteins in both bee lines. The changes in proteome support the general function of hemolymph to drive development and immunity across different ages. However, age-specific proteome settings have adapted to prime the distinct physiology for larvae and adult bees. In larvae, the proteome is thought to drive temporal immunity, rapid organogenesis, and reorganization of larval structures. In adults, the proteome plays key roles in prompting tissue development and immune defense in newly emerged bees, in gland maturity in nurse bees, and in carbohydrate energy production in forager bees. Between larval and adult samples of the same age, RJbs and ITbs have tailored distinct hemolymph proteome programs to drive their physiology. In particular, in day 4 larvae and nurse bees, a large number of highly abundant proteins are enriched in protein synthesis and energy metabolism in RJbs. This implies that they have adapted their proteome to initiate different developmental trajectories and high RJ secretion in response to selection for enhanced RJ production. Our hitherto unexplored in-depth proteome coverage provides novel insight into molecular details that drive hemolymph function and high RJ production by RJbs.
中文翻译：
两种蜜蜂株血淋巴（蛋白质组比较蜜蜂ligustica）显示在驾驶淋巴功能和高蜂王浆分泌发散分子基础
血淋巴对于蜜蜂的免疫力至关重要，并提供了一种调查其生理状态的方法。为了深入了解血淋巴在提高蜂王浆（RJ）产量方面的功能和分子细节，我们对意大利蜜蜂（ITbs）和蜂王浆（RJbs）的幼虫和成年年龄的血淋巴蛋白质组进行了表征和比较。从ITb中选择的库存来增加RJ产量。通过鉴定两种蜂系中的3394血淋巴蛋白，获得了前所未有的深度蛋白质组学。蛋白质组的变化支持血淋巴的一般功能，以驱动不同年龄段的发育和免疫力。但是，针对特定年龄段的蛋白质组设置已经适应了幼虫和成年蜜蜂的独特生理学。在幼虫中，蛋白质组被认为可以促进暂时性免疫，快速的器官发生和幼虫结构的重组。在成年人中，蛋白质组在促进新出现的蜜蜂的组织发育和免疫防御，护士蜂的腺成熟以及觅食蜂的碳水化合物能量产生中起关键作用。在相同年龄的幼虫和成年样本之间，RJbs和ITbs量身定制了独特的血淋巴蛋白质组程序来驱动其生理功能。特别是在第4天的幼虫和哺养蜂中，RJbs中大量的高度丰富的蛋白质丰富了蛋白质的合成和能量代谢。这意味着他们已经适应了蛋白质组学，以启动不同的发展轨迹，并响应于增强RJ生产的选择而高RJ分泌。
更新日期：2017-12-05
中文翻译：
两种蜜蜂株血淋巴（蛋白质组比较蜜蜂ligustica）显示在驾驶淋巴功能和高蜂王浆分泌发散分子基础
血淋巴对于蜜蜂的免疫力至关重要，并提供了一种调查其生理状态的方法。为了深入了解血淋巴在提高蜂王浆（RJ）产量方面的功能和分子细节，我们对意大利蜜蜂（ITbs）和蜂王浆（RJbs）的幼虫和成年年龄的血淋巴蛋白质组进行了表征和比较。从ITb中选择的库存来增加RJ产量。通过鉴定两种蜂系中的3394血淋巴蛋白，获得了前所未有的深度蛋白质组学。蛋白质组的变化支持血淋巴的一般功能，以驱动不同年龄段的发育和免疫力。但是，针对特定年龄段的蛋白质组设置已经适应了幼虫和成年蜜蜂的独特生理学。在幼虫中，蛋白质组被认为可以促进暂时性免疫，快速的器官发生和幼虫结构的重组。在成年人中，蛋白质组在促进新出现的蜜蜂的组织发育和免疫防御，护士蜂的腺成熟以及觅食蜂的碳水化合物能量产生中起关键作用。在相同年龄的幼虫和成年样本之间，RJbs和ITbs量身定制了独特的血淋巴蛋白质组程序来驱动其生理功能。特别是在第4天的幼虫和哺养蜂中，RJbs中大量的高度丰富的蛋白质丰富了蛋白质的合成和能量代谢。这意味着他们已经适应了蛋白质组学，以启动不同的发展轨迹，并响应于增强RJ生产的选择而高RJ分泌。
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